梭梭是荒漠地区主要的先锋植物和建群树种，能够生长在恶劣自然环境下，然而其幼苗对地表高温等逆境胁迫的耐受力相对较差，其分子机制尚未见报道。14-3-3是一类分子伴侣蛋白，参与植物逆境胁迫响应。在本课题组前期克隆梭梭14-3-3基因HaFT-1、HaFT-3、HaFT-9，并发现这3个基因的表达可能与梭梭幼苗对地表高温胁迫的适应性有关的基础上，本项目拟进行以下三方面研究：①梭梭幼苗在受地表高温胁迫和常温复原过程中HaFT-1、HaFT-3、HaFT-9的表达特征，②梭梭幼苗在受地表高温胁迫和常温复原过程中HaFT-1、HaFT-3、HaFT-9的靶蛋白的筛选与鉴定，③HaFT-1、HaFT-3、HaFT-9在高温胁迫响应和常温复原过程中的功能和调控机制。本研究结果将扩展和加深对14-3-3基因在梭梭幼苗适应地表高温胁迫中的作用机制的理解。

Haloxylon ammodendron, which is the main pioneer plant and constructive species in desert areas, can grow in harsh natural environments. However, the ability of tolerance to environmental stresses such as high surface layer temperature (HSLT) stress is relatively poor in H. ammodendron seedlings, and its molecular mechanism has not been reported. 14-3-3 is a kind of molecular chaperon, which is involved in plant stress response. In previous research we cloned H. ammodendron 14-3-3 gene HaFT-1, HaFT-3 and HaFT-9 and found that expression of these three genes was related to adaption to HSLT stress in H. ammodendron seedlings. Based on these results, we will precede the research on HaFT-1, HaFT-3 and HaFT-9 in three aspects in this program: ① expression characteristics of HaFT-1, HaFT-3 and HaFT-9 in the course of HSLT stress and recovery treatments in H. ammodendron seedlings; ② screening and characterization of target proteins of HaFT-1, HaFT-3 and HaFT-9 in the course of HSLT stress and recovery treatments in H. ammodendron seedlings; ③ functional and regulatory mechanism of HaFT-1, HaFT-3 and HaFT-9 in heat stress response and recovery treatment. The results will expand and deepen our understanding on the mechanisms of adaption to HSLT stress in H. ammodendron seedlings.