Bax inhibitor 1（BI-1）是真核细胞中一类关键的凋亡抑制因子，在细胞凋亡的调控中发挥着重要的作用，近期有研究表明BI-1还参与了宿主应对病毒侵染的免疫反应，然而目前还未见鱼类BI-1基因相关功能的研究报道。本项目拟利用石斑鱼脾脏转录组文库中筛选得到的EST克隆石斑鱼BI-1基因，并进一步通过体外过表达、RNA干扰、流式细胞、共聚焦显微镜及免疫共沉淀等技术研究BI-1对虹彩病毒SGIV诱导细胞凋亡的调控作用及其对病毒感染复制的影响，系统地分析BI-1在病毒侵染中的功能与作用机理。研究结果不仅有助于我们进一步了解鱼类在病毒感染过程中的免疫应答机制，而且为鱼类病毒病防治提供新的策略与分子靶标。

Bax inhibitor 1 (BI-1) is a kind of key apoptosis suppressor，play important roles in the regulation of apoptosis. And the involvement of BI-1 in host immune response to virus infection has been proved in recent study. However, the functions of fish BI-1 are still unknown. In this study, we try to clone the grouper BI-1 gene on the basis of EST screened from grouper splenic transcriptome library. Then the effects and mechanisms of grouper BI-1 on SGIV induced apoptosis and virus replication will be investigated through over-expression, RNAi, flow cytometry, confocal microscopy, Co-IP,etc. The results will be beneficial to further understanding the mechanisms of immune responses in fish during virus infection, and provide new strategies and molecular targets for prevention of fish viral disease.