骨质疏松症（OP）发病率高，危害性大，对OP的预防与治疗应当成为研究的热点与重点。目前缺乏能反映出骨质疏松程度的早期诊断试剂盒和价格相对便宜的治疗药物。课题组在前期的研究工作中，已经建立了3000例人群的队列并建立了血清样本库，完成所有人群9种中医体质的调查与资料收集。以往研究证实部分蛋白在OP的诊断及治疗中极具潜力，我们设想还有大量敏感蛋白未被发现，本研究以此为基础，通过蛋白组学的研究方式，使用iTRAQ技术进行研究，旨在从OP患者与健康者血清比较及不同体质间相互比较，探索差异蛋白，将这些差异蛋白与人类国际蛋白指数数据库比对，筛选出敏感蛋白，希望发现能够反映出OP严重程度的特异蛋白，继而开发出有效的OP诊断试剂盒，如果达到研究目的，下一步从差异蛋白中获得能够促进骨形成抑制骨吸收的活性蛋白，将为OP的诊断或治疗提供新的方法，从而产生较大的社会效益与经济效益。

The incidence of osteoporosis (OP) is high and it can causing great harm. Prevention and treatment of OP should become focus and highlights of research. There is currently a lack of early diagnostic kits that reflect the degree of osteoporosis and relatively inexpensive Therapeutic drugs. In the previous research work, we have established a queue of 3,000 people and established a serum sample bank to complete the investigation and data collection of 9 Chinese medicine constitutions for all peoples. Previous studies have confirmed that some proteins have great potential in the diagnosis and treatment of OP. We presume that a large number of sensitive proteins have not been found. Based on Previous study, we use iTRAQ technology and proteomics methods to explore differential proteins from the comparison of serum from OP patients and healthy people, and from different TCM constitutions. Hoping to find specific proteins that can reflect the severity of OP and then to compare these differential proteins with the human international protein index database. Screening out sensitive proteins and then developing effective diagnostic kits for OP. If the research objective is achieved, the next step is to obtain active proteins that can promote bone formation and inhibit bone resorption in differential proteins. This will provide a new method for the diagnosis or treatment of OP and bring in greater social and economic benefits.