砷是一种生物体非必需毒性元素。砷污染是一个全球性问题，其危害在于可以通过农作物进入人体而引发严重的健康风险。随着公众对食品安全的高度关注，如何降低农作物中砷积累成为国家经济和社会发展中迫切需要解决的关键科学问题之一。本项目拟从砷超富集植物蜈蚣草中挖掘砷积累关键基因，用于在水稻中的可控表达，旨在阻断砷向谷粒中的转运和积累。对于基因组信息甚少的蕨类植物蜈蚣草，我们采用新的功能基因挖掘策略，构建了其FOX-Hunting系统（Full-length cDNA Over-eXpressing gene hunting system），本项目将在此基础上，通过表型分析，挖掘关键基因，并对其表达产物进行表达模式分析和亚细胞定位，以揭示砷超富集植物中砷是如何转运并富集于羽叶的分子机制；通过不同启动子控制砷转运关键基因的表达，为抗污染且低积累砷的农作物的分子设计和模块育种提供理论依据和实现途径。

Arsenic (As) is an element that is nonessential for and toxic to organisms. Arsenic contamination in the environment is a global problem, its accumulation in food crops may lead to the serious health risks to humans. With a high degree of public awareness of food safety concerns, how to reduce arsenic accumulation in crops becomes one of the key scientific issues of the economic and social development. This project will discover some arsenic accumulation key genes from a arsenic hyperaccumulator Pteris vittata, and transform to rice to controllable expression, aimed at inhibiting the transport and accumulation of arsenic in the grain. Because little has known about the genomic information in the fern, we have adopted a new strategy of functional gene identification and build its FOX-Hunting System (Full-length cDNA Over-eXpressing gene hunting system). Based on this system, the project will use phenotypic analysis, expression pattern analysis and subcellular localization to reveal the molecular mechanisms of arsenic transport and accumulation in arsenic hyperaccumulator, and to provide some theoretical basis for the molecular breeding of low accumulation of arsenic in crops.