新型转录因子FaRAV1/6介导草莓果实花青苷合成的DNA甲基化调控机制

花青苷积累直接影响果实的外观品质，其合成调控网络正不断被丰富。我们前期研究发现两个新型AP2/ERF转录因子，FaRAV1/6可转录激活花青苷合成主效调控因子FaMYB10，促进草莓果实花青苷合成。RAV成员广泛响应环境刺激，DNA甲基化是植物应答环境重要分子机制，但FaRAV1/6响应环境因子调控草莓果实花青苷合成的DNA甲基化机制仍不是很清楚。本项目在已有研究基础上，以草莓果实为试材，结合光、温度和ABA等处理，采用双荧光素酶、酵母单双杂、凝胶电泳迁移，草莓瞬时表达研究体系，以及基因表达谱和DNA甲基化水平分析，进一步明确FaRAV1/6应答环境因子参与花青苷合成的调控路径及其DNA甲基化机制，以完善花青苷合成的调控网络，为草莓果实外观品质改善提供科学依据。

Anthocyanin accumulation directly affect fruit appearance, and it’s regulation network has been enriching. We identified two novel AP2/ERF, FaRAV1/6, which trans-activate FaMYB10 the master regulator of anthocyanin biosynthesis, and trigger fruit anthocyanin accumulation. RAVs respond to environmental stimuli extensively and DNA methylation functions in the response to environmental stimuli. However, the mechanism of DNA methylation on FaRAV1/6 involved environment stimulated anthocyanin biosynthesis is unclear. Based on previous study and combined with different treatments (light, temperature and ABA), this project aims to elucidate the regulatory pathway, and mechanism of DNA methylation on FaRAV1/6 involved environment triggering anthocyanin biosynthesis through dual luciferase assay, yeast hybrid, EMSA, transient expression system of strawberry, and transcript and DNA methylation analysis. This work will enrich the regulation network of anthocyanin biosynthesis and provide scientific support for improvement of fruit coloration.