DNA methylation mediated by RdDM pathway and demethylation affects furanone accumulation through regulation of QUINONE OXIDOREDUCTASE in strawberry.
DNA methylation mediated by RdDM pathway and demethylation affects furanone accumulation through regulation of QUINONE OXIDOREDUCTASE in strawberry.
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DOI：
10.1093/hr/uhad131
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发表时间：
2023-08
影响因子：
8.7
通讯作者：
中科院分区：
文献类型：
--
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近年来，越来越多的证据表明，DNA甲基化在果实成熟过程中起着至关重要的作用。然而，DNA甲基化在调节特定性状（如风味）中的作用仍不清楚。在这里，我们报告了DNA甲基化在影响草莓呋喃酮生物合成中的作用。草莓醌氧化还原酶（FaQR）是呋喃酮生物合成的关键酶。在草莓品种‘粤新’中有四个FaQR同源物，其中之一FaQR 3贡献了约50%的FaQR转录本，表明FaQR 3在呋喃酮生物合成中起主要作用。通过对果实成熟过程中和施用DNA甲基化抑制剂后的DNA甲基化水平、FaQR 3转录水平和呋喃酮含量的表征，我们发现FaQR 3启动子的DNA甲基化水平与FaQR 3表达和呋喃酮积累呈负相关，表明DNA甲基化可能通过调节FaQR 3表达参与呋喃酮的生物合成，对不同温度的反应一致。此外，RNA指导的DNA甲基化（RdDM）途径中基因FaAGO 4的瞬时表达和24-核苷酸siRNA的富集分析表明，FaQR 3启动子中的DNA甲基化是由RdDM途径介导的。FaDML的瞬时RNA干扰（RNAi）表明去甲基化途径可能参与调节呋喃酮的积累。这些研究结果提供了新的见解的作用，DNA甲基化和去甲基化在影响草莓果实成熟过程中的风味品质。
Recently, increasing evidence suggests that DNA methylation plays a crucial role in fruit ripening. However, the role of DNA methylation in regulating specific traits, such as flavor, remains unclear. Here, we report a role of DNA methylation in affecting furanone biosynthesis in strawberry. Strawberry quinone oxidoreductase (FaQR) is a key enzyme in furanone biosynthesis. There are four FaQR homologs in strawberry cultivar ‘Yuexin’, and one of them, FaQR3, contributes ~50% of FaQR transcripts, indicating a major role of FaQR3 in furanone biosynthesis. Through characterization of levels of DNA methylation and FaQR3 transcript and furanone contents during fruit ripening and after the application of DNA methylation inhibitor, we found that the DNA methylation level of the FaQR3 promoter was negatively correlated with FaQR3 expression and furanone accumulation, suggesting that DNA methylation may be involved in furanone biosynthesis through adjusting FaQR3 expression, and responded to different temperatures consistently. In addition, transient expression of a gene in the RNA-directed DNA methylation (RdDM) pathway, FaAGO4, and enrichment analysis of the 24-nucleotide siRNAs suggested that DNA methylation in the FaQR3 promoter is mediated by the RdDM pathway. Transient RNA interference (RNAi) of FaDML indicated that the demethylation pathway may be involved in regulating furanone accumulation. These findings provide new insights into the role of DNA methylation and demethylation in affecting flavor quality in strawberry during fruit ripening.
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