非O1/O139群霍乱弧菌是人与水产养殖动物共染的病原菌，该菌的流行给水产养殖业带来巨大的经济损失，同时也威胁人类健康。目前有关该菌环境胁迫应激机制尚未阐明，诸如在饥饿等胁迫下，非O1/O139群霍乱弧菌相关功能基因的转录表达发生的变化进而形成胁迫的分子调控网络等许多问题尚未解决。本研究以胁迫反应的主要调控基因rpoS为研究对象，通过缺失株和回补株的构建及其饥饿胁迫后的存活能力、致病力、运动力、形态变化及生物膜的形成等生理生化表型测定，解析rpoS基因与非O1/O139群霍乱弧菌在饥饿胁迫下生存及致病性等生物学表型间的内在关联；从转录水平和翻译水平上验证rpoS基因对T6SS表达、鞭毛以及对生物膜形成等调控相关基因的激活与抑制作用，并揭示rpoS基因在非O1/O139群霍乱弧菌应对环境胁迫而形成的胁迫分子调控网络，为预防和控制由非O1/O139群霍乱弧菌引起的水生动物疾病提供理论支持。

Non-O1/O139 Vibrio cholerae is an important fish and human pathogen. Its prevalence has brought huge economic losses to aquaculture industry and threatened human health. At present, the mechanism about non-O1/O139 V. cholerae stressed by environment have not been clarified, significant changes of transcriptional expression of relevant regulatory genes and stress molecular regulatory networks stressed by starvation have not been solved. The aim of this study was to elucidate the role of the transcription factor RpoS in survival against stresses. The rpoS mutant and complemented strains of non-O1/O139 V. cholerae was constructed by homologous recombination. Survival rates, motility, virulence, morphological changes, biofilm formation of the wild type and the mutant strains under starvation stress were compared. At the transcriptional and translation levels, the role of rpoS on activation and inhibition of TS66, flagellum, biofilm related genes will be clarified. This research will reveal the molecular regulatory networks of rpoS gene in non-O1/O139 V. cholerae survival against stresses and provide theoretical support for prevention and control the diseases caused by non-O1/O139 V. cholerae in aquaculture.