低温春化是植物开花调控的核心途径之一，但木本植物中相关机理的研究几乎是空白。野蔷薇是典型的需低温春化才能开花的植物，而同属的月季开花则不需要春化过程。前期研究表明，模式植物响应低温春化的核心因子FLC在蔷薇科植物中普遍丢失，而同为MADS-Box家族成员的SVP发生了显著扩增。而且，月季中SVP与野蔷薇中SVP相比，丧失了野蔷薇SVP受春化诱导的表达模式。据此推测SVP是调控野蔷薇低温春化的关键基因。本研究以野蔷薇/月季为试材，以是否需低温春化诱导开花为对照表型，通过二者中SVP基因结构、表达模式、表观遗传修饰的差异分析，以及VIGS诱导的基因沉默明确其在野蔷薇低温春化中的功能，再利用转录组测序、表观遗传修饰检测及共表达网络分析构建其调控网络，验证关键节点基因及调控关系，最终阐明SVP调控春化作用及开花的分子机制。相关成果将填补木本植物春化作用的理论空白，为木本植物的花期调控提供理论依据。

Vernalization is one of the most important controller in plant flowering pathways, however, the related researches about the mechanism in perennial woody plants are rare. FLC (FLOWERING LOCUS C) subgroup genes, which act as the key components in the vernalization responses of cruciferous plants, are extensively losing in rosaceous plants. While another branch in the same MADS-Box gene family, SVPs (SHORT VEGETATIVE PHASE) genes are expanded significantly, more and more evidences confirmed their important roles in bud dormancy and dormancy breaking of rosaceous plants. Our previous results showed ‘once flower’ rose Rosa multiflora var. carnea Thory must experience low temperature induced vernalization before flower bud differentiation, in contrast, ‘continuous flower’ rose Rosa chinensis var. Old blush can flower at any favorable conditions without low temperature induction. Furthermore, the expressions of SVPs in Rosa chinensis var. Old blush lost the response to low temperature which exists in Rosa multiflora var. carnea Thory, so the functional differentiation of SVPs in the two species with opposite flowering phenotype implied the crucial roles of SVPs in vernalization. Thus, Rosa multiflora var. carnea Thory and Rosa chinensis var Old blush will be selected as experimental materials, and once flower and continuous flower will be used as a pair of contrastive phenotypes to study the function of SVPs. Firstly, the gene structures, expression patterns and epigenetic modifications of SVPs will be compared in the two species to clarify their differences. Subsequently, VIGS (Virus-induced Gene Silencing) is to be exploited to silence the SVPs in Rosa multiflora var. carnea Thory to determine their function in vernalization. Thirdly, RNA-seq, DNA methylation sequencing, lncRNA (Long Noncoding RNA) sequencing, histone modification examination and co-expression network will be used to construct the regulation network of SVPs, then the roles of the key genes on the net nodes and the regulation relationships will be proved, thus, the function of SVPs in vernalizations and flowering of Rosa multiflora and their regulation molecular mechanism will be achieved. The expected results will fill the gaps of the vernalization theory for perennial woody plants, and provide the theoretical basis for flowering time regulation of woody plants.