胃肠道间质瘤(GIST)术后5年内的复发转移率高达50%。我们在前期研究中发现转录因子Slug的表达与GIST的转移密切相关，靶向沉默Slug基因能有效抑制GIST细胞的侵袭转移，但在机体内Slug如何参与GIST的转移并不清楚。本项目拟利用生物信息学技术和miRNA芯片筛选出GIST中Slug调控相关的miRNAs；通过基因表达调控实验、双荧光素酶报告系统进行验证。然后分析miRNAs与GIST临床病理特征的关系；检测 miRNAs对GIST细胞侵袭转移能力的影响及并探讨其中的分子机制。最后利用整体荧光成像技术在活体内实时监测miRNAs对GIST原位移植瘤生长转移的影响。本项目旨在鉴定Slug调控相关的miRNAs，探讨miRNAs/Slug影响GIST转移的分子机制，并评估应用外源性miRNAs抑制GIST细胞侵袭转移的可行性，为GIST的治疗提供新的思路。

The recurrence and metastasis rate of gastrointestinal stromal tumors (GISTs) is up to 50%. Our previous studies have shown that Slug correlated closely with metastasis of GISTs,silencing of Slug gene impaired invasiveness of GISTs cells in vitro. However, it's not clear that how Slug takes role in regulating metastasis of GISTs. In this project, the miRNAs which might take a role in regulation of Slug expression will be predicted by bioinformatics technique, screened by miRNA microarray tests, and verified by dual luciferase reporter assay and regulation of gene expression experiment. Subsequently, the corressions of miRNAs with clinicopathologic characteristics of GIST patients will be analyzed. The influence of Slug and miRNAs on invasive and metastasis ability of GIST cells, and the related mechanism will be detected. Finally, the influence of miRNAs and Slug on growth and metastasis of GIST xenograft tumor will be detected by whole-body fluorescence imaging. This project aims to identify miRNAs that regulate slug expression and to investigate the molecular mechanisms of their influence on GISTs metastasis. Furthermore, this project aims to assess the feasibility of using exogenous miRNAs to inhibit invasion and metastasis of GIST cells, which will provide a new idea for the treatment of GISTs.