黄曲霉毒素B1（AFB1）污染范围广，毒性强，严重威胁人类和动物健康。在单胃动物体内，AFB1需经肝细胞色素P450酶系转化形成环氧化物而发挥其强毒性及致癌性，反刍动物体内研究尚处于空白，其代谢激活及导致肝脏损伤的机理尚不明确。本项目拟以奶山羊为模型动物，基于转录组学、代谢组学、实时荧光定量PCR和免疫印迹等分子生物学技术，评估日粮AFB1暴露对反刍动物消化代谢、泌乳性能、肝脏氧化应激和细胞凋亡的影响，揭示其在反刍动物体内的转化和代谢规律，筛选可用于诊断AFB1中毒的血、奶、尿液生物标记物，明确参与AFB1代谢激活的P450酶亚型，探索AFB1诱导奶山羊肝细胞凋亡的分子机制。研究结果有助于系统评估饲料AFB1对反刍动物的危害，为建立反刍动物AFB1中毒快速诊断技术，以及通过添加外源物质或采取其他手段减少AFB1在反刍动物肝脏内的代谢激活并改善其诱导的细胞凋亡紊乱奠定理论基础。

Aflatoxin B1 (AFB1) is a potent hepatotoxic and hepatocarcinogenic mycotoxin which could contaminate various foods and feeds and pose great health risks to humans and animals. In monogastric animals, AFB1 is primarily bioactivated by hepatic cytochrome P450 enzymes to highly toxic and reactive genotoxic metabolites known as AFB1-8,9-epoxide. However, the exact mechanisms of AFB1 metabolism in ruminant liver are still unclear. This study aims to identify the P450 enzyme orthologs involved in the bioactivation of AFB1 in dairy goat hepatocytes and expression pattern of apoptosis related genes by using RNA-Seq technique combined with RT-qPCR and Western blot analysis. The underlying molecular mechanisms of AFB1 bioactivation and the induced apoptosis will be further investigated. Moreover, effects of dietary AFB1 on the nutrient digestion and metabolism, lactation performance, hepatic oxidative stress and apoptosis will be explored. The expected results of this project will provide toxicological data for systematically evaluating the heath risks of AFB1 on ruminants and help to develop new strategies for minimizing the bioactivation of AFB1 and the related liver injury by means of genetic breeding, nutrition, feeding technology and management, etc.