中医理论认为甘草能解百药毒，近代对甘草的研究主要集中在配伍减毒方面，但解毒作用机制尚未完全阐明。本课题组前期对"基于转运体对甘草与有毒中药的配伍减毒机制"，及马钱子主要成分在中枢的毒代动力学展开了系列研究，发现甘草通过上调转运体的功能和表达，促进中枢毒物外排的解毒机制，但仍难以解释甘草快速解毒的原因。本项目结合文献和前期研究基础，提出"甘草能快速解毒可能与其某种/些效应物质直接作用于靶部位有关"的假说。选择有多器官毒性的马钱子为模型毒物，SD大鼠为实验动物，采用微透析取样和LC/MS/MS在线检测分析技术，建立解毒剂量甘草已知成分在不同靶器官的PK-PD关系，及其可测定活性成分群与各靶点药效学之间的ΣPK-ΣPD结合模型，评估各主要成分的疗效贡献度，构建甘草在不同靶器官的"解毒指数"，以验证该假说。对阐明甘草解毒的体内效应过程，揭示甘草解毒的物质基础，探索甘草解毒的潜在机理提供科学依据。

According to traditional Chinese medicine theory, glycyrrhiza can detoxify hundreds of drugs. In modern times, studies on glycyrrhiza mainly focused on the compatibility to reduce the poison, but the detoxification mechanism had not been fully clarified. Our preliminary work is as follows: the detoxicating mechanism of glycyrrhiza when in combination with poisonous TCM based on transportor and the toxicokinetic study in Semen Strychni's major gradient of brucine and strychnine . The results showed that glycyrrhiza can promote the detoxification mechanism of central poison efflux through the function and expression of upregulated transportor, but it is still hard to explain the reason why glycyrrhiza can rapidly detoxify.Based on literature and previous research foundation, we proposed the hypothesis that detoxicating mechanism of glycyrrhiza is related to some effective substances which work on the tagret site directly.Semen Strychni with multiple organ toxicity is selected as the poison model, and microdialysis sampling and LC/MS/MS online detection and analysis techniques are used. PK-PD of known ingredients of glycyrrhiza with detoxification dose under different target organs are established, curative effects are evaluated for various major absorptive components, and "detoxification index" of glycyrrhiza under different target organs are constructed. They all used to analyse and verify the hypothesis.It provides a scientific basis to clarify in-vivo effect process of glycyrrhiza detoxification,reveal the material base and explore the potential mechanism of glycyrrhiza detoxification.