蜈蚣草是世界上被发现的第一种砷超富集植物，对砷具有极强的吸收萃取能力，因此被广泛应用于砷污染土壤的植物修复。然而，截至目前，蜈蚣草高效吸砷的分子机制并不清楚。作为磷的类似物，砷主要通过磷酸盐转运蛋白Pht1被植物吸收。本项目前期通过转录组测序和基因挖掘，克隆得到了3个蜈蚣草砷吸收相关的Pht1基因：Pht1;3，Pht1;4和Pht1;5。基于此，本项目将以不同Pht1为研究对象，通过分析不同Pht1酵母转化株砷含量，验证其对砷的转运能力；将不同Pht1基因导入模式植物拟南芥中，分析其对拟南芥砷吸收、转运和积累的影响，解析其在植物砷吸收中的功能；采用原位杂交、GFP融合蛋白等技术手段，明确不同Pht1在蜈蚣草中的组织定位和亚细胞定位特征，并结合其在酵母和拟南芥中的功能，分析其在蜈蚣草高效砷吸收中的作用，为提高砷污染土壤植物修复效率提供重要基因资源。

Pteris vittata, the first arsenic hyperaccumulator identified, has very strong ability to absorb the arsenic, so it is widely used in phytoremediation of arsenic contaminated soil. However, the molecular mechanism of arsenic uptake efficiency of Pteris vittata is not clear. As a phosphorous analogs, arsenate is absorbed mainly by the phosphate transporter Pht1. Using the transcriptome sequencing and gene cloning, three members of Pteris vittata Pht1, Pht1;3, Pht1;4 and Pht1;5, have been identified. In this proposal, we will verify their arsenic transport capacity through the analysis of arsenic content in different Pht1 strains of yeast transformation lines; analyze absorption, translocation and accumulation of arsenic in Pht1 expression Arabidopsis lines; identify tissue specific expression pattern and subcellular localization, using insitu hybridization and GFP fusion protein, respectively. Combing the expression pattern, subcellular localization characteristics and functional analysis in yeast and Arabidopsis, the function of the Pteris vittata Pht1 in arsenate absorption will be identified, which will contribute to improving the efficiency of phytoremediation of arsenic contaminated soils and provide important genetic resources.