黄灯笼辣椒是颇具海南地方特色的辣椒种类，但近年来遭受甜椒脉斑驳病毒（PVMV，Potyvirus）等的危害日趋严重。PVMV基因组编码的融合蛋白在病毒蛋白酶作用下产生11种成熟蛋白，其中包含两个分子量为6kDa的蛋白—6K1和6K2。先前我们研究表明：PVMV和该属李痘病毒（PPV）的6K1蛋白在相应病毒感染细胞中形成点状内含物，与病毒复制复合体（VRC）核心组份共定位于叶绿体和细胞核的周围区域（Potyvirus病毒复制场所），是VRC的重要组份。但有关6K1蛋白如何被招募至病毒复制位点以及6K1与VRC相互作用的分子机制尚未明确。本研究我们采用PVMV/本氏烟（黄灯笼）致病系统，综合利用生物化学、分子生物学和细胞生物学等技术手段，鉴定寄主或病毒蛋白在招募6K1至病毒复制位点以及介导6K1/VRC互作中的功能作用。预期研究结果为设计新颖的黄灯笼辣椒抗PVMV策略提供新思路。

Capsicum chinense is well known as one local specialty chilli in Hainan, and currently it has being severely damaged by pepper veinal mottle virus (PVMV), one member in the genus Potyvirus. The +ssRNA genome of PVMV encodes two polyproteins that are proteolytically processed by three viral proteinases into 11 mature proteins, including two molecular weight of 6-kDa proteins 6K1 and 6K2. Previously, we demonstrated that either PVMV or Plum pox virus (PPV, Potyvirus) 6K1 protein formed punctuate inclusions in presence of viral infection, were associated with core components of viral replication complex (VRC), and colocalized in the periphery of chloroplasts and nuclei, in which potyviral replication was believed to occur. However, the molecular mechanism is largely unknown about how 6K1 protein is recruited to viral replication site and interact with VRC. In this study, the pathosystem of PVMV/Nicotiana benthamiana (C. chinense) is employed to address the above questions. In brief, host or viral proteins interacting with 6K1 protein will be screened and identified, and their potential roles in recruiting 6K1 protein to viral replication site and mediating 6K1/VRC interaction will be dissected. The anticipated results will provide new insights to designing novel strategies of PVMV resistance in C. chinense.