特应性皮炎是临床上常见的慢性瘙痒性炎症性皮肤病，发病年龄低，病程长，目前尚缺乏治愈或长期缓解的治疗药物，探索安全有效的治疗方法及其作用机制一直是相关领域的热点科学问题。镇心安神方是基于《内经》“诸痛痒疮，皆属于心”理论指导下形成的治疗新思路，在前期应用本方治疗特应性皮炎取得可靠疗效基础上，提出“镇心安神方可能通过调控GRPR/MrgprA3/TRPs信号通路来阻断瘙痒信号传导”的假说。本研究拟以Nc/Nga小鼠为动物模型，通过行为学观察、体内及体外实验，采用免疫荧光、ELISA、Western bolt、Real-time PCR等检测技术，研究镇心安神方对通路相关细胞、蛋白、分子及基因表达的影响，探索镇心安神方缓解特应性皮炎慢性瘙痒的疗效及其分子机制，为“从心神论治”特应性皮炎及其它瘙痒性疾病提供科学依据。

Atopic dermatitis (AD) is a common skin disease characterized by pruritus, chronically relapsing inflammatory and early occurrence. Currently, it is still lack of durgs which can cure or relief the disease. Seeking for the safe and effective treatment of AD has been a hot scientific issues in related fields. According to the theory of " All kinds of diseases with pain, itching and sore are exclusively related to the heart " referred in neijing, Formulation for Tranquilizing Heart (FTHM), as a new method, is used for the treatment of AD. In our previous research, FTHM was efficacious and safe for AD patients. We suppose that FTHM relieves pruritus by restraining GRPR/MrgprA3/TRPs signaling pathway. In this study, we intend to evaluate the pruritic behaviors in Nc/Nga mice, simultaneously, use immunofluorescence technique, ELESA, Western blot and Real-time PCR methods to test associated protein, molecular and mRNA expression of signaling pathway through vivo and vitro experiments. Through the above studies, we expect to reveal the molecular mechanism of FTHM relieving chronic itching of AD, and provide the scientific basis for the theory of "Treatment determination from heart and mind " in AD and other pruritic diseases.