肿瘤转移是胆囊癌患者的主要死亡原因，外泌体在肿瘤转移中扮演重要角色，而外泌体在胆囊癌转移中的作用机制尚不清楚。我们前期研究发现TCTP与胆囊癌的转移密切相关，并且发现TCTP存在于高转移潜能胆囊癌细胞外泌体中，富含TCTP的外泌体能显著促进低转移潜能胆囊癌细胞侵袭转移，结合已有的研究证实双青蒿素能结合TCTP并促进其降解。据此我们提出假说：高转移潜能胆囊癌细胞通过外泌体将TCTP传递给低转移潜能胆囊癌细胞，从而增强后者的侵袭转移能力。利用双青蒿素抑制外泌体中TCTP，可望达到抗击胆囊癌转移的目的。本课题拟在深入阐明高转移潜能胆囊癌细胞源性外泌体促进胆囊癌侵袭转移分子机制的基础上，进一步挖掘潜在的干预性手段，寻找抗击胆囊癌转移的有效治疗手段，改善胆囊癌患者的预后。

Tumor metastasis is the main cause of death in patients with gallbladder carcinoma. Exosomes play an important role in tumor metastasis. The mechanism of exosomes in the metastasis of gallbladder carcinoma remains unclear. In our previous study, TCTP was associated with the metastasis of gallbladder carcinoma and existed in the exosomes of high metastatic potential gallbladder cancer cell. Exosomes rich in TCTP could significantly promoted the invasion and metastasis of low metastatic potential gallbladder carcinoma cells. Some studies have confirmed that Dihydroartemisinin can bind TCTP and promote TCTP degradation. Based on this, we hypothesize that high metastatic potential gallbladder carcinoma cells deliver TCTP to low metastatic potential gallbladder carcinoma cells through exosomes, thereby enhancing the latter's metastatic potential. It is expected to achieve the goal of combating gallbladder cancer metastasis by using Dihydroartemisinin to inhibit TCTP. This project intends to further clarify the molecular mechanisms of high metastatic potential gallbladder carcinoma cell exosomes promoting gallbladder carcinoma invasion and metastasis and tap potential interventions to find effective ways to combat gallbladder cancer metastasis, thus to improve gallbladder cancer patients' prognosis.