运动纤毛分布于人体呼吸道、脑室等上皮细胞表面，它们之间的协调运动是机体排出粘液、细菌等异物和促进脑液循环的前提，但控制多纤毛协调运动的机理还不清楚。本项目以模式生物衣藻的鞭毛运动协调失衡突变体为研究材料，根据其突变基因（bcd1）编码鞭毛轴丝辐条蛋白并含有血红素结合结构域的特点，将利用酵母双杂交和BioID等技术鉴定鞭毛静止和协调运动时与BCD1互作的蛋白；将利用电镜和蔗糖梯度离心等技术研究BCD1在辐条中的精细定位及其在辐条形成中的作用；将利用代谢组学等方法检测鞭毛中血红素的含量和组成，以及与BCD1结合在纤毛协调运动中的作用。将建立基于荧光蛋白的鞭毛内氧化还原态实时检测方法，阐明纤毛协调运动与鞭毛内氧化还原动态变化的关系。最后，利用突变体和分离的核-基体-鞭毛复合物，构建调控鞭毛协调性运动的鞭毛内氧化还原通路。该研究将为阐明运动纤毛异常所致纤毛病的成因以及相应纤毛病的治疗提供理论支持。

Motile cilia are widely distributed on the epithelial cells of tracheal and encephalocoele, and their coordinated beating is the premise for transporting the mucus and bacteria out of human body and circulating the fluid in encephalocoele of brain. However, the mechanism of coordinated beating of motile cilia is unclear. We will take advantage of the beating coordination mutants obtained from Chlamydomonas, a model of classic motile cilia research. Based on that one of the mutated genes encoded a heme-binding radial spoke proteins (BCD1), we will identify the interacting proteins of BCD1 using the yeast two hybridization and BioID techniques，and determine the localization of BCD1 in radial spoke and the roles of BCD1 in assembling the radial spoke complex using the EM and sucrose gradient centrifugation methods. Then, we will determine the amount of heme in flagella and the functional significance of the binding the BCD1 with heme in coordinating the beating of multi-cilia. In addition, we will set up an in vivo method to quantify the dynamics of redox in flagella and illuminate the relationship between oscillation of redox in cilia and beating coordination of motile cilia. Finally, we will reconstitute the redox regulation pathway involved in the coordination of ciliary beating by using the mutants and isolated nucleus-basal body-flagella complex. These works will make great contribution in understanding the formation of motile cilia related ciliopathies and shed new light in treating these diseases.