纤毛/鞭毛分布在大多数真核细胞的表面，具有运动和感受功能。纤毛的形成和解聚依赖鞭毛内运输机制Intraflagellar Transport (IFT)。IFT包含由Dynein-2驱动的IFT-A复合体和kinesin-2驱动的IFT-B复合体，目前认为IFT-A将纤毛内的蛋白运出纤毛，而IFT-B 将蛋白由细胞内运入纤毛，但纤毛内所有蛋白是否都依赖IFT运输、被IFT运输的货物在纤毛中的运动特点以及IFT运输蛋白货物的机理都不清楚。本项目将利用定量蛋白质组学的方法全面地鉴定被IFT运输的货物；研究被IFT-A和被IFT-B运输的货物在纤毛中的运动特点；将运用荧光共振能量转移(FRET)等方法验证申请人提出的两种IFT与货物相互作用的假说："携带假说"和"泛素标签假说"。这些研究将为阐明IFT与货物相互作用的调控打下基础， 有利于了解纤毛在不同细胞中富集特殊的蛋白分子的机理。

Cilia/flagella are hair-like organelles emanating from the cell surface of most of eukaryotic cells. Their assembly and disassembly are dependent on Intraflagellar Transport (IFT). IFT is composed of IFT-A complex and IFT-B complex, IFT-A is driven by a microtubule minus motor dynein-2 and transports the turnover products out of cilia, IFT-B is motivated by a plus microtubule motor Kinesin-2 and moves the axonemal precursors into the cilia, but the mechanism of the IFT in transporting the cargos is unknown. We plan to identify all of the cargos of IFT by using the quantitative proteomic approach and analyze the motility behavior of the putative cargos using the FRET method. We proposed two models: "Carrying Model" and "Ubiquitin-labeling Model" and will testify these models by using the Fluorescence Resonance Energy Transfer (FRET) and Immunoprecipitation (IP) methods. These works will speed our understanding the regulation of the interaction between the IFT and cargos and the mechanism of cilia enriching a subset of proteins in different types of cells.