过继T淋巴细胞免疫治疗是辅助治疗胶质瘤术后残余病灶最具前景的方法之一。提高过继T淋巴细胞肿瘤归巢能力并实现T淋巴细胞的无创性示踪以探索免疫治疗的机制是亟待解决的问题。生物素受体在肿瘤细胞表面过量表达，可作为靶向生物标志实现肿瘤的靶向性免疫治疗。多模态高驰豫纳米材料——NaGdF4-TAT-FITC是理想的磁共振T1纳米示踪剂，同时可实现荧光显像。本研究拟在体外将生物素与T淋巴细胞耦合，并以TAT介导NaGdF4标记T淋巴细胞后尾静脉注射入小鼠脑胶质瘤模型，使标记淋巴细胞以生物素作为靶向探针与胶质瘤生物素受体特异性结合。本研究的目的为提高淋巴细胞肿瘤识别能力的同时通过MR/荧光实时示踪，实现靶向性T淋巴细胞胶质瘤归巢的可视化，并研究其在胶质瘤生物素受体靶向免疫治疗中的机制及作用规律。该项目对指导细胞免疫进行脑胶质瘤残余肿瘤的个体化生物治疗有重要意义。

T lymphocyte adoptive therapy may be the most prospect method for complete cure of the remnant lesion of glioma.The development of tumor-specific delivery systems for T lymphocyte and efective method for tracking tumor-specific T lymphocyte in vivo are two urgent needs for efficacious immunotherapy. Biotin recptor is overexpressed on tumor cells and it could ba a promising tumor targeting marker in immunotherapy. A multi-modal high-relaxivity nanomaterial----NaGdF4-TAT-FITC is a ideal MR T1 nanoprobe, as well as a fluorescence probe.Our study inject biotinylated T lymphocyte which are labeled with NaGdF4-TAT-FITC to the body of glioma mice via vein. As a targeting probe, the biotin on lymphocyte will specifically bind to biotin receptor on tumor cells, improving tumor identification capability of lymphoma and realizing real-time tracking in vivo with MRI and fluorescence optical imaging. We will realize the visualization of targeted T lymphocyte in biodistribution,tumor homing and treatment effect and assess the feasibility and mechanism of biotin-receptor as a glioma treatment target site.This item will be of important research and clinical meaning to guide private biological treatment on remnant glioma with cellular immunotherapy.