六溴环十二烷(HBCD)是一组含多种立体异构体的新型溴代阻燃剂，主要有(±)α-，β-，γ-HBCD六种。它们具有持久性有机污染物的特性，能在海洋生物体内富集，并对水生生物有很强的毒性，但它们之间是否在毒性效应和毒理机制上有差异还未被报道过。另外HBCD对海洋生态系统重要一环- - 海洋桡足类的的毒性也鲜见报道。本项目拟以中国近海代表性海洋桡足类- - 浮游性中华哲水蚤和底栖性日本虎斑猛水蚤为研究对象，立足于比较HBCD主要异构体 [(±)α-，β-和γ-HBCD] 对海洋桡足类的毒性效应差异、揭示它们在桡足类体内进行代谢转化的主要途径以及所导致的解毒或增毒机制，并进一步进行蛋白质组学分析，以探讨HBCD在海洋桡足类中的毒性作用机制和生物体对其的应激反应机制。本研究项目有助于更全面认识HBCD异构体在海洋生物中的毒害作用，为评估HBCD对海洋生态系统造成的潜在影响提供科学依据。

Hexabromocyclododecane (HBCD), one of the most widely used brominated flame retardants,includes a group of diastereoisomers and the major ones are (±)α-，β-，γ-HBCD. They are persistent in the environment, toxic to the aquatic organisms and bioaccumulative in marine animals. It is unknown that whether there is difference in the toxicity and toxic mechanism among the diastereoisomers. Moreover, the toxicity of HBCD to marine copepods, one of the most important parts in marine ecology, is still not well understood. Here we propose to use the representive copepod species of Chinese coastal area, i.e., calanoid copepod Calanus sinicus and harpacticoid copepod Tigriopus japonicus, to test the toxicity of different HBCD stereoisomers, reveal the major bioisomerization and biotransformation pathways for detoxification or intoxication, and expolore the possible toxic mechanisms by proteomics approach. The results of this study will shed important light on the understanding of HBCD toxicity to marine organisms and provide important information for marine environmental risk assessment of HBCD.