小麦孢囊线虫是世界范围内分布的禾谷类作物重要病原线虫。目前已经对我国的麦类作物生产构成了严重威胁，严重制约着麦类作物生产的发展。由于传统诊断方法的制约性，研发新的诊断速度更快、成本更低、信息量更大的诊断技术是进行该病害准确测报和有效防控的基础，且具有重要的理论和实践意义。本项目拟以经济上危害最大、形态上最难区分的H.avenae和H.filipjevi作为研究对象，运用SCAR标记、Real-time PCR和LAMP技术研究小麦孢囊线虫的早期快速准确检测和诊断的分子生物学方法，开发检测罹病植物小麦组织和土壤中H.avenae和H.filipjevi单头线虫的检测技术，研发出简便易行的田间诊断检测试剂盒，以实现尽早检测和防控，满足基层科技工作者病害测报和防控的需求。

Wheat cereal cyst nematodes are the destructive pests to cereal crops and have spread all over the world. They seriously threaten and restrict the development of cereal crops production in China. The traditional diagnosis of wheat cereal cyst nematodes is inefficient, developing new technology with less time-consuming, less cost and more information will lay a foundation for accurate and effective diagnosis of wheat cereal cyst nematodes. Using H. avenae and H. filipjevi as materials, the scientific research item goes in for developing early and rapid molecular diagnostic technique of them through SCAR marker, Real-time PCR and LAMP. And this molecular diagnostic technique will be able to detect and diagnose signal H. avenae and H. filipjevi exiting in wheat root tissue and soil, which will realize early detection and control of them. Finally, basing on this molecular diagnostic technique, we will develop a rapid diagnosis and detection kit to satisfies local scientific and technical personnel needs of the pests prediction and control.