WH1fungin环脂肽是来源于解淀粉芽胞杆菌的一种Surfactin（表面活性素）类生物表面活性剂，经研究发现它是一种安全性良好，具有较强免疫佐剂活性的天然产物，可诱导巨噬细胞产生ROS，促进IL-1β释放，与炎症小体的激活密切相关。本项目在前期研究基础上，从细胞及分子水平上进一步探索WH1fungin佐剂激活炎症小体的作用机理。主要研究内容：WH1fungin体内外刺激巨噬细胞，检测WH1fungin是否可以激活巨噬细胞炎症小体及类型；非变性SDS-PAGE、热电泳及圆二色谱法检测WH1fungin与炎症小体NLRP3、NLRP1、IPAF、AIM2人源重组蛋白相互作用；siRNA转染THP-1分化的巨噬细胞，检测WH1fungin可能作用的炎症小体受体。本项目的开展有助于更好地了解Surfactin类佐剂作用机制，为Surfactin的进一步应用奠定基础，为其开发成疫苗佐剂提供参考。

Cyclic lipopeptide WH1fungin, one kind of Surfactin biosurfactants which was isolated from the culture of Bacillus amyloliquefaciens WH1, was found to be with characteristics of well security and strong immune adjuvant activity in previous studies, WH1fungin could induce ROS produced and IL-1β released in macrophages, which was related with inflammasome activated. Based on the previous studies, we explored the mechanism of WH1fungin activating inflammasome. WH1fungin whether could activate inflammasome and which types of inflammasome could be activated, We will detect WH1fungin whether could interact with human inflammasome by non denatured SDS-PAGE, Thermal electrophoresis, circular dichroism in vitro and by siRNA transfaction of macrophages differentiated from THP-1 cell. The study of the mechanism of WH1fungin activating inflammasome in macrophages will not only help to better understand the mechanism of adjuvant of Surfactin, but also for further application of Surfactin, and could provide references of its development into a vaccine adjuvant.