长链多不饱和脂肪酸对三疣梭子蟹卵巢成熟过程中脂肪蓄积影响及其机理的研究

脂类作为壳类动物重要的能量来源和必需脂肪酸，特别是长链多不饱和脂肪酸，参与整个性腺发育过程。本研究以三疣梭子蟹为试验对象，首先在三疣梭子蟹卵巢发育组织学变化分期的基础上，利用代谢组学的技术确定发育各阶段的代谢特征产物，通过测定卵巢发育各期三疣梭子蟹血淋巴中脂蛋白、卵巢和肝胰腺脂类类别和脂肪酸组成以及脂肪积累相关基因表达，研究不同长链不饱和脂肪酸日粮对三疣梭子蟹发育各期卵巢的脂肪蓄积的影响。进而应用体外器官培养模型研究长链脂肪酸与三疣梭子蟹卵巢吸收与利用的关系，从而揭示长链不饱和脂肪酸、卵巢发育与脂肪蓄积三者之间的关系。阐释长链不饱和脂肪酸调控三疣梭子蟹卵巢发育过程中脂肪蓄积的分子机制，完善三疣梭子蟹脂类营养的理论，并为三疣梭子蟹卵巢成熟以及优质亲体培育提供理论依据。

Dietary lipids are considered as an important source of energy and essential fatty acids (EFA) for the crustaceans and long-chain polyunsaturated fatty acids（LC-PUFAs）have also been shown to be of special significance for gonad maturation and brood quality. In this proposed study, we take the swimming crab Portunus trituberculatus as an experimental model. Metabolomics techniques will be utilized to determine the metabolic characteristics in the course of ovary maturation of swimming crabs. Lipoproteins, lipid class and fatty acid profiles will be characterized in the hemolymph, ovary and hepatopancreas, we will conduct to investigate metabolism related genes expression. We will also investigate the effects of different dietary long-chain polyunsaturated fatty acids on Portunus trituberculatus ovarian development and lipid accumulation. We will take advantages of the in vitro organ culture system to study how long-chain polyunsaturated fatty acids affect ovarian lipid absorption and utilization. Understanding the disparity in FA disposal between ovary and lipid accumulation may reveal mechanisms involved in the regulation of long-chain polyunsaturated fatty acids on ovarian development and lipid deposition in Portunus trituberculatus. This pioneering work will pave the road for future research along this line and will provide the basis for understanding Portunus trituberculatus lipid nutrition, gonad maturation and for improving brood quality.