由于随排水系统进入污水处理厂污泥的纳米颗粒会显著抑制微生物活性、进而制约污泥厌氧消化系统的高效运行，因此开展纳米颗粒对厌氧消化效能抑制机制的研究十分必要。本项目以不同粒径的纳米CuO、ZnO、AgNO3为研究对象，拟采用静态吸附实验、荧光标记-激光共聚焦显微等测试手段，深入分析不同粒径纳米颗粒在污泥胞外聚合物(EPS)上的附着行为、形态特征及析出规律，阐明该过程与污泥微生物絮体细胞结构破坏的相关性；借助于DNA高通量测序技术，揭示典型纳米颗粒对厌氧消化系统微生物活性抑制和微生物群落结构影响的作用机制，进而解析污泥有机物可能的代谢途径；结合XAD树脂分级技术，构建消化系统中纳米颗粒特性、污泥EPS组分去除特征、微生物活性间的三维构效关系，获取纳米颗粒存在时污泥厌氧消化系统稳定运行的评判指标。该研究成果可为典型纳米颗粒存在条件下污泥厌氧消化系统的高效运行提供理论依据和技术支撑。

Worldwide application of nanotechnology lead to an increasingly release of nanoparticles in wastewater, which potentially impairs the operation the anaerobic digestion of sewage sludge. Thus, research of the inhibition of nanoparticles on the biomass in sludge digestion is therefore of great significance. To clarify the inhibition mechanisms of typical nanoparticles (CuO, ZnO, AgNO3) on the sludge digestion, batch adsorption tests and multiple fluorescence labeling/confocal laser scanning microscope (CLSM) were applied to study the attachment characteristics of nanoparticles on the sludge extracellular polymeric substances (EPS) firstly, and to analyze the correlation between the attachment methods and its corresponding negative effect on the chemical structure of biomass. Secondly, high-throughput sequencing was used to clarify the changes of the microbial groups and biomass activities in the digestion system, to reveal the negative effects of nanoparticles on the metabolic pathways of the sludge organics. Moreover, by using the XAD fractionation technology, an three-dimensional structure-activity relationship among the EPS fractions reduction, the biomass activity and the characteristics of nanoparticles in the anaerobic digestion system was constructed. Finally, the index for poor operation of digestion system was also built under the condition of nanoparticels additive. The investigation will provide theoretical basis as well as technical support for the steady-state operation of digestion, which could deal with the negative effect of typical nanoparticles.