磺胺类抗生素生物毒性显著且在土壤中易累积，污水厂二级出水渗滤过程中此类物质广泛存在影响着渗滤系统的高效运行。针对渗滤系统长期运行中磺胺类抗生素如何抑制功能微生物活性，并影响消毒副产物(DBPs)前驱物去除这一尚待探索的科学议题，本项目以磺胺甲恶唑、磺胺吡啶、磺胺嘧啶、磺胺甲嘧啶、磺胺二甲嘧啶为研究对象，首先通过人工/序批式渗滤实验，辅以电化学、色谱/光谱技术，深入分析其在渗滤系统累积机理、生物有效性特征及降解途径；基于渗滤系统微生物群落分布和抑菌活性实验，借助DNA高通量测序，阐明抗生素对渗滤系统中碳/氮营养物质代谢相关功能微生物的毒性阈值和致毒机理；在此基础上揭示抗生素抑营养物质代谢下碳系、氮系DBPs生成特征；通过入渗DBPs前驱物去除限制性因子分析和出水生物毒性实验，提出渗滤所得再生水风险调控措施。研究成果可为渗滤所得再生水中抗生素/中间产物、DBPs有效控制提供理论依据和技术支撑。

Sulfonamides antibiotics in secondary effluent would significant restrict the operational efficiency of infiltration systems, for their high ecotoxicity and significant accumulation rate in soil. However, the inhibition mechanism of those sulfonamides on the biomass activities and subsequent negative effect on the removal of disinfection by-products precursors during the infiltration systems operation was still unclear. To clarify the characteristics of the accumulation, bioavailability and biodegradation pathways of typical sulfonamides (sulfamethoxazole, sulfadiazine, sulfapyridine, sulfamethazine, sulfamethazine) during secondary effluent reclaiming, lab-scale soil column/sequential column recharging systems will be constructed and the operational performance are analyzed based on electrochemical analysis, spectrum analyzing, as well as batch adsorption tests. Secondly, negative effects of those accumulated sulfonamides on the distribution/activity of microbial groups (related to C-, N-nutrients biodegradation) would be evaluated via high-throughput sequencing, and the corresponding toxicological threshold values of those sulfonamides be emphatically analyzed. Subsequently, variation of C-, N-DBPs (disinfection by products) formation potential under the C-, N-nutrients removal inhibition are deeply evaluated. Finally, ecotoxicological characterization of the formed DBPs, sulfonamides, and their intermediates within the obtained reclaimed water will be evaluated. Combined with the analyzing of the rate limiting factors for DBPs precursors removal, as well as the chem/biotoxicity assessment, control measures of sulfonamides and DBPs induced ecotoxicity of the reclaimed water from recharging system are summarized. The investigation will provide theoretical basis as well as technical support for the steady-state operation of secondary effluent recharging system, which could deal with the negative effect of typical antibiotics.