各种致病因素导致的胆道损伤是肝移植术后的主要并发症之一，也是影响移植后患者生存和再次肝脏移植手术的最主要原因，发生率在20-30%之间；移植术后由于免疫抑制抑制剂的使用，患者处于免疫低下状态，感染是肝移植术后胆道损伤的重要因素之一，然而很多细菌或病毒应用现有的临床培养等技术无法获得病原学证据。本课题应用宏基因组测序技术，通过基因组学和代谢组学分析探索肝移植术后胆道中致病微生物种类、基因表达和代谢通路，阐明胆道微生态异常导致的移植肝胆道损伤的机制，为临床肝移植术后胆道损伤的进一步诊治奠定基础。

Bile duct injury makes the major complication of liver transplantation, which affects survivals after the procedure, and also the main cause of re-transplantation. The incidence of bile duct injury is between 20%-30%, and infetion plays an important part especially in patients taking immunosuppressive agents. However, the available clinical laboratory technology could not provide etiology evidence for most bacteria or virus infection. In this subject, META-genomis sequencing technology is used to explore the species, gene expression and metabolic pathways of microbes by the means of genomics and metabonomics anylisis. To elucidate the mechanisms of how biliary microbiota abnormalities injures the bile duct would lay a foundation of diagnosis and treatment of bile duct injury after liver transplantation.