种子细胞启动组织再生过程并募集更多自体细胞参与。DFCs是牙周组织工程常用种子细胞，来源有限，如何解决并诱导更多自体细胞参与牙周组织再生极为关键。项目前期发现DFCs与BMSCs共培养可能更利于牙周组织再生，LIPUS也具有协同作用，但LIPUS如何协同DFCs与BMSCs诱导牙周组织再生的作用不清楚。本研究拟在前期国家自然科学基金研究基础上，采用组织工程、分子生物学等实验方法，构建DFCs/BMSCs共培养体系和相应细胞、动物模型，研究DFCs/BMSCs共培养下细胞相互作用对增殖、诱导分化的影响，观察对自体细胞的募集效应，探讨LIPUS增强细胞功能活性、细胞间相互作用，促进细胞募集，抑制LPS介导的牙周组织局部炎症，促进牙周组织再生等生物学机制。项目紧密结合组织工程技术和无创理念，以期阐明DFCs/BMSCs联合应用和LIPUS的协同效应在牙周组织再生的作用，为牙周组织再生开辟新思路。

Seed cells start the process of tissue regeneration, then recruit massive autologous cells to participate in this regeneration. However, as the common seed cell of periodontal tissue engineering, Dental Follicle Cells(DFCs) show poor resources; Hence, having further numerous autologous cells recruited in the periodontal tissue regeneration is the key element. Our previous studies have showed that the co-culture of DFCs and Bone Marrow Mesenchymal Stem Cells(BMSCs) might make for tissue regeneration, and Low-Intensity Pulsed Ultrasound(LIPUS) provides synergistic effect on this process. However,the exact mechanism involved is still undefined. Based on our previous Natural Science Foundation of China(NSFC) research, we construct the co-culture system of DFCs/BMSCs and related animal models to analyze the proliferation and differentiation of DFCs and BMSCs cell-interaction in the co-culture , and to observe cell homing of autologous cells ,with the technology of tissue engineering and molecular biology. Furthermore, we’ll investigate the exact molecular mechanism of biological events referring to LIPUS enhancing the activity of seed cells, boosting cell-interaction, inducing cells homing, suppressing the LPS-meditated periodontal local inflammation and promoting the periodontal tissue regeneration. Our team combine this project with the tissue engineering technology and the medical philosophy of noninvasive ;We intend to determine the role of the cell-interaction played in the co-culture of DFCs and BMSCs. Morever, we’ll investigate the combined application of DFCs/BMSCs and the synergistic effect of LIPUS during periodontal tissue regeneration. The research shall devote a brand new thought for the periodontal tissue regeneration.