本项目以申请者已成功发展的痕量植物激素（赤霉素、油菜素甾醇）创新分析方法、自动样品前处理装置、实际植物样品测试应用为基础，针对植物学基础研究的微量样品、原位实时以及同时检测痕量植物激素的现实需求，提出开展微量样品中痕量植物激素的超高灵敏分析技术及装置研究。项目从植物激素分析检测的全流程出发，创新植物样品萃取机理、制备高选择性萃取材料、筛选高效质谱增敏衍生试剂；综合提高样品前处理的绝对回收率、萃取纯化后样品的上样利用率、质谱离子化效率，从而降低样品使用量20-30倍。本项目将实现亚毫克植物样本中赤霉素等酸性激素同时检测，以及毫克级植物样本中油菜素甾醇的定量检测，适用于微量实验室样本和原位实时取样样本分析，并研制一套微量样品自动样品前处理装置，在中科院植物研究所做应用示范。从而为中国植物学基础研究，特别是植物激素分析，提供先进、可靠、自主的检测分析技术和装置。

Based on novel analytical methods for trace plant hormones of gibberellins (GAs) and brassinosteroids (BRs), an automated sample preparation instrument, and applications on real plant samples, developed in our group, we propose an project to develop an in-situ & on-site sampling technologies of ultra-sensitive analysis of trace plant hormones in milligram samples with simultaneous determination of multi-components trace hormones, in order to meet with current challenges in fundamental plant research. Arising from the whole plant hormone analytical process, which consisted of sample preparation, LC separation and MS detection, these microextraction methods will be further developed and optimized. Study on hydrophilic and selective adsorbents, and high sensitive derivation reagents will be developed and screened for GAs and BRs. Combining these factors together, the minimum sample amount needed for trace hormones analysis will be decreased by 20-30 folds. The goals of this project are to simultaneously analyze GAs and other acidic plant hormones with the sample less than one milligram, to quantify BRs with sample less than ten milligrams, and to upgrade the automated sample preparation instruments for milligram samples. These methods and instruments can be applied for micro-size plant samples and the specimens collected in-situ & on-site from live individual plant or specific tissue. The instrument will also be demonstrated in Institute of Plant, CAS. Generally, this project will contribute to basic plant research in China, especially in trace plant hormone analysis of micro sample, with advanced, reliable, and independent methods and instruments.