目前，单以遗传学说难以解释不断升高的变应性疾病发病率而使表观遗传调控受到极大关注。区域淋巴结作为调控局部免疫应答的枢纽未引起足够重视，本研究基于Tregs在诱导变应性鼻炎（AR）免疫耐受中的关键作用拟通过表观遗传修饰Tregs经区域淋巴结诱导变应性鼻粘膜的免疫耐受，以期寻找治疗AR的新方法。本课题研究内容：①通过Massarry、RT-PCR等方法检测AR中Tregs表观遗传修饰及对下游免疫应答的调控；②检测PM2.5、PM10等环境因素对Tregs关键因子foxp3甲基化/去甲基化的影响；③利用去甲基化剂体外诱导变应原特异性Treg细胞foxp3去甲基化；④通过动物模型探讨表观遗传修饰后的Tregs经区域淋巴结诱导免疫耐受并观察其子代的表观遗传现象。以期从表观遗传学角度解释AR的发病及与环境污染的关系，从而探讨表观遗传调控Tregs诱导免疫耐受在AR治疗中的作用和潜在临床可行性。

The sharp increase in allergic disease prevalence over the past 2 or 3 decades is difficult to explain by genetics,so epigenetics as a mechanism mediating gene-environment interaction got great attention. Regional lymph node acting as the hub of the local immune response should be highly valued.This project is based upon the key role of Tregs in inducing allergic rhinitis (AR) immune tolerance,plans on utilizing Tregs modified by epigenetics to inducing immune tolerance of allergic nasal mucosa via regional lymph node in order to find new method of treatment of AR. Concrete research content include: ①analyze the epigenetic modification of Tregs in AR by using Massarry, RT - PCR and the downstream immune response regulation.②detect the correlation between some environmental factors ,such as PM2.5、PM10,and the methylation/demethylation of foxp3 which is the key factor of Tregs.③induce foxp3 demethylation of allergen specific Tregs in vitro by using demethyl-agent.④allergic animal models will be performed to test whether epigenetics modified Tregs could induce immune tolerance of allergic nasal mucosa after regional lymph node injection and then detect epigenetics phenomenon of its daughter. The object is explaining the pathogenesis of AR from epigenetics perspective and its correlation with environmental pollution,thereby we will address future challenges and opportunities for the new field,focusing on those that might have potential clinical therapeutic role in preventing allergic disease.