疼痛是困扰帕金森病（Parkinson's disease , PD）患者常见的非运动症状之一，可出现于运动症状之前，也是导致早期PD被误诊的重要原因之一。目前PD相关疼痛的诊断率低，治疗无统一标准。关于PD相关疼痛机制的研究也较少，确切机制不明。多巴胺能药物只能改善部分PD患者疼痛，提示多巴胺能以外的系统也参与了PD相关疼痛的调节。我们的前期研究发现在6-OHDA诱导的PD大鼠模型中，延髓头端腹内侧5-HT能神经元受损、投射到脊髓背角的5-HT能神经纤维以及5-HT含量减少。5-HT3A受体抑制剂可以缓解PD模型大鼠的疼痛。本研究将在此基础上，运用分子生物学及电生理等技术，结合药理学方法研究不同亚型5-HT受体对PD疼痛的下行易化作用，深入研究脊髓背角中5-HT3受体介导Ca2+/CaMKII信号通路参与PD相关疼痛的机制。本项目的研究将为明确PD相关疼痛机制提供理论依据。

Pain is one of the most common non-motor symptoms in patients with Parkinson's disease (PD). It can occur before motor symptoms and is also one of the major causes of misdiagnosis of early PD. The rate of the diagnosis of PD-related pain is low. There is no uniform treatment standard for pain in PD. There are also few studies on the pathogenesis of pain in PD. The exact pathogenesis mechanism of pain in PD is unknown. Dopaminergic treatment can only relieve pain in some of the PD patients, suggesting that systems other than dopaminergic system, are also involved in the regulation of pain in PD patients. Our previous study found impaired 5-serotonin (5-HT) neurons in the rostral ventromedial medulla in the 6-OHDA-induced PD rat model and decreased 5-HT nerve fibers and 5-HT content projected to the spinal dorsal horn. 5-HT3A receptor inhibitors can relieve pain in PD model rats. Based on this, we will use molecular techniques and electrophysiological techniques to study the facilitating effect of 5-HT receptors on PD related pain in combination with pharmacological methods. We will further use 6-OHDA-induced PD rat model to study the role of 5-HT3 receptors mediated Ca2 + / CaMKII Signaling involved in PD-related pain in the spinal dorsal horn. The research of this project will provide a theoretical basis for clarifying the pathogenesis mechanism of pain in PD.