目前，人们对既具有光学成像又能增强MRI成像乃至能装载药物的多功能纳米粒子表现出很大兴趣，因这种纳米粒子在诊疗一体化领域有重要应用前景。而现今此类多功能纳米粒子都是通过复合不同功能的粒子来制备的，如将荧光量子点与MRI成像对比剂进行复合制备出具有荧光和磁共振双模式成像的纳米粒子。本研究致力于设计合成类普鲁士蓝化合物等纳米粒子，研究结构单元中金属离子替换，-C≡N-配位方式的异构化对磁共振成像T1和T2的影响规律，研究Mn2+离子的4T1-6A1跃迁荧光波长受配位环境改变的影响规律；利用类普鲁士蓝化合物纳米粒子的天然结构孔洞和大比表面积，探讨其在抗癌药物阿霉素（DOX）装载方面的应用，致力于制备出具有T1-T2 MRI成像和荧光成像，兼有药物装载能力的多功能纳米粒子，通过连接靶向剂和对皮下癌种植模型鼠肿瘤靶向治疗及监测治疗效果方面的研究，评估其多功能应用的可能性。

Multifunctional Nanoparticles with magnetic resonance and fluorescence dual modal imaging, and drug loading as well have aroused much attention due to their potential applications in clinical diagnosis and treatments. A wide range of molecules and nanoparticles have been investigated as agents for contrast enhanced MRI and fluorescence imaging in cancer diagnosis. However, a single material with dual modal imaging of MRI and TPF is rarely reported. Here, we aim to design and prepare M3[Co(CN)6]2 Prussian blue analogue MOF nanoparticles which can serve as agents for both T1- and T2-weighted MRI, fluorescence imaging and DOX carriers. Tumor cells are used to establish the tumor in immunodeficient mice which will be used for in vivo imaging and delivering targeted therapy.