卵巢储备功能下降继而引起卵巢早衰容易诱发多囊卵巢综合征和围绝经期综合征，严重影响女性生育能力及生活质量。我们在课题组以往研究工作基础上，以早期生长反应基因（EGR-1）和芳香化酶P450基因表达为切入点研究金雀异黄素（GEN）对不同年龄段雌性大鼠不同发育期卵泡的调节作用，以期明确其作用机制。拟选择70日龄和12月龄雌性大鼠作为研究对象，分别模拟育龄期和围绝经期女性，给予不同剂量的GEN，取卵巢组织及细胞以完成下列实验内容：1. GEN对卵巢储备功能的调节作用；2.GEN对EGR-1的调节作用；3. GEN对雌激素生成关键酶芳香化酶p450及其编码基因CYP19基因以及雄激素合成关键酶StAR、P450scc的调控作用。目前国内外关于植物雌激素对卵巢早衰调节作用的研究还未见系统报道，因此，针对GEN对不同发育期雌性大鼠卵巢储备功能及卵巢早衰的调节作用及机制研究具有重要意义。

Descent of ovarian reserve function and premature ovarian failure is facility to cause polycystic ovary syndrome and perimenopausal syndrome, which has an serious impact on female fertility and quality of life. In order to clarify the mechanism, we study the regulation effects of genistein on different developmental stages of follicles through the expression of early growth response factor (EGR-1) and aromataes P450. In order to study whether the estrogen like effects of genistein (GEN) can regulate ovarian function and delay the aging of ovary or not, on the basis of previous research of our research group, we would selecte 70 days age and 12 months age female rats as the research objects, which analogue childbearing age and perimenopausal women. The animals were given a certain dosage of GEN and the ovaries tissues and cells obtained in order to complete the experiments: 1.Effect of GEN on ovarian apoptosis regulatory factors; 2.Effect of GEN on EGR-1; 3.Effect of GEN on aromatase P450, CYP19 gene and StAR、P450scc; Reports of the effect of plant estrogens on premature ovarian failure still has no systematic report, therefore, it has important significance to study regulation effects and mechanisms of genistein on ovarian reserve function and premature ovarian failure in female rats.