溶解性有机质对水环境中典型PPCPs的生物有效性的影响研究

溶解性有机质（DOM）对PPCPs生物有效性的影响研究对于评价真实水环境中PPCPs的生态环境风险具有十分重要的意义。本项目以PPCPs的自由溶解态浓度为核心，以生物吸收和毒性效应为主体，开展了以下四个方面的研究：（1）建立了三种新型被动采样技术（极性化学物整合采样POCIS、一次性极性纤维微耗损固相微萃取nd-SPME和基于离子液体的微耗损中空纤维膜液相微萃取nd-HF-LPME）以采集不同性质PPCPs的自由溶解态浓度；（2）基于自由溶解态浓度指示化学有效性，考察了不同来源DOM对PPCPs化学有效性的影响及作用机制；（3）基于自由溶解态浓度以及生物吸收和毒性效应，揭示了DOM对PPCPs生物有效性的影响及机制，尤其是DOM结合态PPCPs的贡献；（4）以鱼体血液中PPCPs为内暴露浓度，以鱼体胆汁中母化合物及葡萄醛酸化代谢产物为暴露性生物标志物，进一步揭示生物有效性影响机制。

Studies on the impacts of dissolved organic matter (DOM) on the bioavailability of pharmaceuticals and personal care products (PPCPs) are very important and significant to evaluate the eco-environmental risks of PPCPs in the real aquatic environment. Using the freely dissolved concentrations (Cfree) of PPCPs as core and the biouptake and toxic effects as main body, the following four aspects of research will be conducted. (I) Three novel passive sampling techniques including polar organic chemicals integrated sampler (POCIS), disposable polar fiber negligible depletion solid phase microextraction (disposable nd-SPME) and ionic liquid based negligible depletion hollow fiber supported liquid phase microexecration (IL-nd-HF-LPME) will be developed for sensing the Cfree of various PPCPs. (II) Using Cfree as indicator of chemical availability, the effects of DOMs from different origins on the availability of PPCPs will be studied and the interaction mechanisms will be elucidated. (III) Based on Cfree and biouptake and toxic effects of PPCPs, the effects of DOM on the bioavailability of PPCPs will be investigated and the interaction mechanisms will also be discussed, especially the contribution of the DOM-associated PPCPs. (IV) Using the plasma PPCP concentrations in fish as the internal concentrations and the bile concentrations of the parent PPCPs and their corresponding glucuronidation metabolites as biomarkers, the bioavailability mechanisms will be further discussed.