藻源细胞结合有机质溶出特征及碘代有机物生成和毒性效应研究

藻源细胞结合有机质（COM）作为藻源有机质（AOM）的重要组成部分，在碘代有机物(I-OCs)生成过程中具有十分重要的作用。本研究以COM为中心，考察消毒条件和模拟自然条件下I-OCs的生成机制和毒性效应，并将COM与AOM的其它组分及天然有机质进行比较。具体如下：（1）结合多种分析方法，考察COM在极端条件和自然条件下的溶出和结构特征。（2）考察氯化消毒（氯和氯胺）和环境相关过程（光化学和二氧化锰氧化）中I-OCs的生成机制：采用高分辨质谱对未知I-OCs进行识别和鉴定，结合传统I-OCs和未知I-OCs分析，考察COM作为前驱物生成I-OCs的机制。（3）结合灵敏的活体毒性（斑马鱼行为学）及传统的离体细胞毒性，以及生成I-OCs的综合分析，研究毒性效应与I-OCs物质组成的关系，综合评价I-OCs的毒性效应。本研究为藻源水体中I-OCs的控制和生态环境风险评价提供理论基础和技术支持。

As an important part of algogenic organic matter (AOM), cellular-bound organic matter (COM) plays an important role in the formation of iodinated organic compounds (I-OCs). However, at present, our understanding of COM is extremely lacking, and the contribution potential and mechanism of COM as a precursor to I-OCs formation is still in the exploration stage, so this is a new scientific problem that needs to be studied urgently. In this project, focusing on COM, the formation mechanism and toxic effect of I-OCs under disinfection and simulated natural conditions will be investigated, and these aspects of COM will be compared with other components of AOM and natural organic matter (NOM). The details are as follows: (1) The dissolution and structural characteristics of COM under extreme and natural conditions will be investigated by combining with a variety of analytical methods. (2) Based on the analysis of traditional I-OCs and unknown I-OCs by high-resolution mass spectrometry, the mechanism of I-OC formation by COM as precursor will be investigated, especially the formation mechanism in chlorination (chlorine and chloramine） and environment related processes (photochemistry and manganese dioxide oxidation). (3) Combined with sensitive in vivo toxicity (zebrafish behavior) and traditional in vitro cytotoxicity, as well as known I-OCs and unknown I-OCs analysis, the relationship between toxicity effect and I-OCs composition was studied, and the toxicity effect of I-OCs was comprehensively evaluated. This study provides theoretical basis and technical support for the control and ecological environment risk assessment of I-OCs in algae source water.