基于类核孔蛋白多肽的人工细胞核孔复合体

真核细胞核孔复合体是细胞核与细胞质之间物质运输的通道，具有极高的选择性和分离速率。本项目拟将核孔蛋白的高选择透过性与传统高分子材料的工程性质相结合，通过嵌段共聚物自组装模拟细胞核孔复合体的结构与功能。主要研究内容包括：（1）精确可控合成基于类核孔蛋白多肽的新型嵌段共聚物；（2）研究嵌段共聚物在本体和薄膜中的自组装行为，模拟细胞核膜的结构特征；（3）探索生物分子在薄膜中的运输，模拟细胞核膜的功能。本项目的完成将提供一种简易可行的自下而上的方法，用合成材料来制备具有高度选择透过性的智能分离膜。这类材料有望应用于药物递送、分子过滤、生物信号响应等多个领域。

Nuclear pore complexes are the transport channels across the nuclear envelope of the eukaryotic cell nucleus, with very high selectivity and transport rate. In this proposal, we are going to mimic the structure and functions of the nuclear pore complex via self-assembly by integrating the selectivity of nucleoporins and mechanical properties of polymers. This project will focus on: (1) precision synthesis of the nucleoporin-like polypeptide based block copolymers; (2) self-assembly behaviors of these block copolymers in bulk and thin film states, mimicking the structural characteristics of the nuclear pore complex; and (3) the selective transport of biomolecules, mimicking the functions of the nuclear pore complex. This project will provide a facile bottom up approach to prepare smart separation membranes with high selectivity using synthetic materials, which could be applied in many areas such as drug delivery, molecular filtration, biosensor, and others.