盆底支持结构中胶原蛋白和弹性蛋白的减少是盆底功能障碍性疾病（PFD）发生的关键因素。理论上，骨髓间充质干细胞（BMSCs）可促进盆底组织的再生和修复，使PFD注射治疗成为可能，但需提高BMSCs局部生存和分化为成纤维细胞并促进其分泌胶原蛋白和弹性蛋白的能力。我们前期研究证实富血小板血浆（PRP）凝胶可作为BMSCs的支架，同时有利于细胞的粘附和增殖。为长期提高BMSCs有效分化为成纤维细胞，课题组成功研制了能长期平稳释放bFGF的纳米缓释系统。在前期研究基础上，本研究拟进一步以elastin基因转染BMSCs，在PRP凝胶条件下，与包封bFGF的纳米缓释微粒一起，通过自主研制的搅拌注射器混匀并注射至PFD大鼠模型的盆底薄弱部位，使薄弱部位生成的成纤维细胞大量分泌胶原蛋白，同时亦能较多分泌弹性蛋白，并最终促进盆底支持组织的再生和修复，为PDF的早期、微创治疗提供新的途径。

Pelvic floor dysfunction (PFD) is a common gynecological disease. Traditional surgery or using graft to reconstruct pelvic floor has its limitations. Studies show a close relationship between collagen as well as elastin in pelvic support structure and PFD. The application of bone marrow mesenchymal stem cells (BMSCs) to promote tissue regeneration and repair of the pelvic floor shows promising results to the treatment of PFD. The key to this new treatment is to increase BMSCs’capability of differentiation to fibroblast and facilitate the secretion of collagen and elastin by fibroblast. Our previous study found that platelet-rich plasma (PRP) gel can not only be used as scaffold for BMSCs but also can improve the cell adherence and proliferation. Basic fibroblast growth factor (bFGF) can act as cytokine to induce BMSCs. The controlled release nano- microspheres invented in our previous study can effectively stabilize and release bFGF slowly. In this study, to improve BMSCs’ the capability of differentiation to fibroblast and facilitate the secretion of collagen and elastin by fibroblast, the elastin gene modified BMSCs will be mixed with PRP gel and injected into the weak spot of rat PFD model together with the bFGF encapsulated in the controlled release nano- microspheres by the stirring syringe invented by us, which will help the generated fibroblast to secrete collagen and elastin and then ultimately enhance the pelvic floor tissue regeneration and repair. This therapy could provide a new none-invasive treatment on the PFD.