MicroRNA-29 facilitates transplantation of bone marrow-derived mesenchymal stem cells to alleviate pelvic floor dysfunction by repressing elastin.
MicroRNA-29 facilitates transplantation of bone marrow-derived mesenchymal stem cells to alleviate pelvic floor dysfunction by repressing elastin.
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MicroRNA-29促进骨髓间充质干细胞移植，通过抑制弹性蛋白缓解盆底功能障碍
DOI：
10.1186/s13287-016-0428-7
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发表时间：
2016-11-17
影响因子：
7.5
通讯作者：
Hua X
中科院分区：
文献类型：
--
作者：
Jin M;Wu Y;Wang J;Ye W;Wang L;Yin P;Liu W;Pan C;Hua X
骨盆底功能障碍（PFD）是一种影响全球许多女性的疾病，症状包括压力性尿失禁（SUI）和盆腔器官脱垂（POP）。我们之前已经证明了稳定表达弹性蛋白的骨髓间充质干细胞（BMSC）在大鼠中减轻了PFD，并旨在进一步研究microRNA-29 a-3 p调节对弹性蛋白表达和BMSC移植治疗效果的影响。我们抑制BMSCs中内源性microRNA-29 a-3 p，并通过RT-PCR和Western blot研究其对弹性蛋白表达的影响。然后将MicroRNA-29抑制的BMSC移植到PFD大鼠中，伴随着使用聚（乳酸-共-乙醇酸）（PLGA）纳米颗粒（NP）中配制的bFGF持续释放bFGF，然后进行尿动力学试验评价。MicroRNA-29 a-3 p抑制导致BMSCs体外培养中弹性蛋白的表达和分泌上调。将负载PLGA的bFGF NP共注射到PFD大鼠体内后，microRNA-29 a-3 p抑制的BMSCs显著改善尿动力学测试结果。我们的多学科研究，结合microRNA生物学，基因工程BMSC和纳米颗粒技术，为修复结缔组织和治疗PFD提供了一种出色的干细胞治疗方法。
Pelvic floor dysfunction (PFD) is a condition affecting many women worldwide, with symptoms including stress urinary incontinence (SUI) and pelvic organ prolapse (POP). We have previously demonstrated stable elastin-expressing bone marrow-derived mesenchymal stem cells (BMSCs) attenuated PFD in rats, and aim to further study the effect of microRNA-29a-3p regulation on elastin expression and efficacy of BMSC transplantation therapy. We inhibited endogenous microRNA-29a-3p in BMSCs and investigated its effect on elastin expression by RT-PCR and Western blot. MicroRNA-29-inhibited BMSCs were then transplanted into PFD rats, accompanied by sustained release of bFGF using formulated bFGF in poly (lactic-co-glycolic acid) (PLGA) nanoparticles (NP), followed by evaluation of urodynamic tests. MicroRNA-29a-3p inhibition resulted in upregulated expression and secretion of elastin in in vitro culture of BMSCs. After co-injection with PLGA-loaded bFGF NP into the PFD rats in vivo, microRNA-29a-3p-inhibited BMSCs significantly improved the urodynamic test results. Our multidisciplinary study, combining microRNA biology, genetically engineered BMSCs, and nanoparticle technology, provides an excellent stem cell-based therapy for repairing connective tissues and treating PFD.
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影响因子：
2
作者：
Goldman, Howard B.;Sievert, Karl-Dietrich;Damaser, Margot S.
通讯作者：
Damaser, Margot S.
影响因子：
5.3
作者：
Li SH;Sun Z;Guo L;Han M;Wood MF;Ghosh N;Vitkin IA;Weisel RD;Li RK
通讯作者：
Li RK
DOI：
10.1073/pnas.0805038105
发表时间：
2008-09-02
影响因子：
11.1
作者：
van Rooij, Eva;Sutherland, Lillian B.;Olson, Eric N.
通讯作者：
Olson, Eric N.
影响因子：
4.1
作者：
Ulrich, Daniela;Edwards, Sharon Lee;Gargett, Caroline E.
通讯作者：
Gargett, Caroline E.
影响因子：
2.3
作者：
Zhou Y;Ling O;Bo L
通讯作者：
Bo L