肝再生是各种肝损伤后肝脏恢复正常功能的基础，如何促进肝再生是临床亟待解决的重要问题。新近研究发现，HO-1能够促进肝再生，但其具体作用机制尚不清楚。肝窦内皮细胞（LSECs）介导的肝窦重建及血管新生是功能性肝再生的关键环节。我们前期发现，HO-1对维持LSECs自身稳态和功能的发挥具有重要作用；在肝再生早期，HO-1能促进肝内血管生成和肝细胞增殖，同时HO-1能上调损伤肝脏VEGF的表达。而VEGF-VEGFR2-Id1信号轴的激活是保障LSECs促肝再生功能发挥的关键途径。据此，我们推测HO-1可能通过调控LSECs的VEGF-VEGFR2-Id1信号轴促进肝再生。本项目选择LSECs为靶细胞，以HO-1为切入点，通过体外培养LSECs和HO-1基因敲除、敲入小鼠70%肝切除体内实验，探讨HO-1对LSECs促肝再生的作用及具体机制，为临床上HO-1促进肝再生提供理论依据。

Liver regeneration is the basis for the liver to restore to physiological function after various liver injuries. How to promote liver regeneration is an important clinical problem to be solved. Recent studies have found that HO-1 can promote liver regeneration, but the mechanisms of HO-1 functions are still unclear. Liver sinusoidal endothelial cells (LSECs) mediated hepatic sinus reconstruction and angiogenesis are key role in functional liver regeneration. Our previously studies found that HO-1 plays an important role in maintaining the homeostasis and function of LSECs. In the early stage of liver regeneration, HO-1 can promote angiogenesis and hepatocyte proliferation, and HO-1 can up-regulate the expression of VEGF in injured liver. Activation of the VEGF-VEGFR2-Id1 signal axis is a key pathway to ensure that LSECs promote liver regeneration. Based on these results, we hypothesized that HO-1 may promote liver regeneration by regulating the VEGF-VEGFR2-Id1 signal axis of LSECs. In this study, we will aim at LSECs，and regard HO-1 as the target point，the effects of HO-1 on liver regeneration in LSECs were investigated by in vitro culture of LSECs and in vivo HO-1 knockout and knockin mice 70% hepatectomy model. And the mechanisms provide a theoretical basis for clinical treatment of HO-1 promoting liver regeneration