胰岛素样生长因子（Insulin-like growth factor, IGF）信号通路在调控生理生长和营养代谢中发挥重要功能。IGF结合蛋白（IGF binding protein, IGFBP）是调控IGF信号通路活性的关键因子，但目前关于水产动物IGFBP蛋白家族的功能研究较少，阻碍了对IGF信号通路生理及营养调控功能的认识。因此，本项目以重要经济鱼类大菱鲆作为研究对象，系统阐述养殖鱼类IGFBP蛋白家族的功能，研究内容包括：（1）揭示IGFBP蛋白家族的时空表达模式；（2）分析IGFBP蛋白家族调节大菱鲆肌细胞IGF-TOR信号通路活性和细胞增殖的机制；（3）体内实验论证IGFBP/IGF复合体活性对大菱鲆生理生长和蛋白质营养的调控作用。本项目研究成果将为以IGF信号通路为靶点进行水产动物精准营养调控提供理论依据。

Insulin-like growth factor (IGF) signaling pathway is the main regulatory signaling pathway for fish physiology and growth, it also plays critical role in the regulation of nutrition and metabolism. IGF binding protein (IGFBP) family is the key molecule to regulate the activity of IGF signaling pathway. However, the research on the function of IGFBP in aquatic animals is still rare, which hinders the understanding of the physiological and nutritional regulation function of IGF signaling pathway. Using turbot, an important economic fish, as the animal model, we will systematically describe the function of IGFBP protein family in cultured fish. The research contents include: (1) determining the spatiotemporal expression pattern of IGFBP; (2) revealing the regulatory mechanism of IGFBP on IGF/TOR signal pathway and cell proliferation and apoptosis of turbot muscle cells; (3) evaluating the effects of IGFBP / IGF complex activity on the physiology, growth, and protein utilization of turbot. The research results of this project will provide theoretical basis for precise nutrition regulation of aquatic animals with IGF signal pathway as the target.