ICC细胞是自发性电活动的起源，是膀胱起搏细胞。既往发现针刺通过抑制ICC细胞兴奋性、降低胞内游离Ca2+浓度、抑制逼尿肌缝隙连接蛋白Cx43等途径，调节逼尿肌不稳定收缩，证实针刺调节ICC细胞兴奋及其信息通讯是治疗不稳定膀胱的肌源性机制之一。晚近发现，ICC细胞不仅具有起搏功能，且是胆碱能神经兴奋向逼尿肌传递的中转站。如何从ICC细胞传递和整合胆碱能神经/肌源性信号机制中，进一步证实针刺调节效应和作用靶点？本研究以逼尿肌过度活动、逼尿肌无力为对象，借助免疫荧光双标法、激光共聚焦技术、膜片钳技术等，研究针刺对膀胱ICC细胞与胆碱能神经结构关系、ICC细胞胆碱能受体、ICC细胞起搏电流及T型钙通道亚型等影响，结合尿动力变化，分析胆碱能受体功能与ICC细胞兴奋及膀胱收缩变化相关性，揭示针刺影响ICC细胞传递胆碱能神经信息功能及调节ICC细胞兴奋性机制，阐述针刺双向调节逼尿肌收缩功能的科学理论。

ICC are the origin of spontaneous electrical activity and are the pacemakers of the bladder cells. Past studies have found that acupuncture can inhibit the excitability of ICC in the bladder, reduce the free Ca2 ion concentration in the cells, inhibit detrusor Cx43 gap junction, and adjust the detrusor unstable contraction. They confirmed that the mechanism of acupuncture treating overactive bladder is through adjusting the excitability and communication of bladder ICC. Recent studies found that bladder ICC not only have the pacemaker functionality, but are also the transfer station between cholinergic nerve and the detrusor. From the mechanism of ICC transmission and integration of cholinergic nerve/myogenicity, how do we prove that acupuncture is able to regulate and pinpoint the effective target? The objects of this research are overactive detrusor and adynamia of detrusor. With double-label immunofluorescence assays, laser confocal microscopy, and patch clamp techniques, we can study how acupuncture affect the bladder ICC and cholinergic nerves structure, the ICC cholinergic receptors, and on ICC pacemaker currents and T type Calcium tunnel subtype. To analyze the function of cholinergic receptors and the relationship between ICC excitability and bladder contraction, we combined with urodynamics to reveal the mechanism of how acupuncture effects ICC cholinergic nerve transmission and ICC excitability regulation. This research demonstrates the scientific theories of acupuncture’s bi-directional regulation connected with the detrusor excitability.