类风湿性关节炎是一种慢性进行性的自身免疫性疾病，其治疗及机理是目前国内外研究的热门课题，但迄今未有理想的治疗药物成熟和面世应用。蜂针作为中医药治疗类风湿性关节炎的众多疗法中较为独特的一种，止痛、消炎、防止关节继续变形的作用非常明显。我们之前的研究已经证明了蜂针治疗类风湿性关节炎的获效机理是通过对细胞因子的免疫调控和抑制滑膜细胞的异常凋亡而发挥作用的。但由于蜂针的过量应用会出现过敏反应，所以能发挥最佳治疗作用的蜂针使用剂量和时间的探讨就显得尤为重要。本研究以大鼠佐剂性关节炎法建立免疫性炎症模型，通过关节滑膜组织病理形态学及超微结构、相关细胞因子及细胞凋亡指标的检测和观察，探讨不同蜂针剂量和蜂针治疗时间与疗效的关系，并对其进行安全性评测。通过以上研究，确定蜂针最佳安全治疗剂量和疗程，为蜂针治疗类风湿性关节炎最佳临床方案的制定提供有力实验依据。

Rheumatoid arthritis（AD） is a sort of chronic progressive autoimmune disorder. It's treatment and pathogenesis is a popular topic at present. However, up to now there has not been the best medicine to cure AD. Traditional Chinese medicine incloud numerous therapies to treatment it. Bee-acupuncture therapy is a distinct one to relieve pain eliminate inflammation and prevent joints deformed. We have proved bee-acupuncture therapy could treatment AD through immunoregulating cytokine and inhibiting anomalo- apoptosis of synovial cell. But overdose of bee-acupuncture coule cause anaphylactic response. Therefore the research about the best dose and time of bee-acupuncture to cure is very important. These reseach is an experimental study on the dose-effect chronergy relationship and safety of bee-acupuncture therapy to rheumatoid arthris model rats through detecting and observing pathomorphological fine structure of synovial tissue of joint and index of cytokine apoptosis. We could define the best safe therapeutic dose and course of treatment though these research. We could provid powerful vidence to institute the best clinic scheme of curing AD.