前期研究证实前列通窍胶囊可促进良性前列腺增生（BPH）大鼠膀胱逼尿肌细胞凋亡，并明显降低BPH大鼠膀胱逼尿肌中NGF、bFGF表达。文献报道BPH所致的持续性膀胱出口梗阻（BOO）可以造成膀胱血流下降和缺氧，而前列通窍胶囊是否具有调节BOO后膀胱血流和改善其缺氧状态的作用正是该项研究要解决的关键科学问题。为进一步揭示该药的作用靶点和机制，采用聚丙烯缝线将SD大鼠前列腺部尿道进行部分结扎以造成BOO大鼠模型后灌胃给药，利用荧光微球技术检测膀胱血流，采用胶原酶消化法分离培养SD大鼠膀胱逼尿肌细胞，通过缺氧小室建立缺氧模型，用前列通窍胶囊含药血清干预，采用免疫组化、Western Blot、RT-PCR等技术检测细胞活性及HIF、VEGF、TGF-β、TNF-α等在膀胱中的表达，以此揭示该药保护BOO后膀胱功能的部分作用机制，为进一步开发治疗BOO所致膀胱功能障碍的中药新药奠定理论基础。

Previous study demonstrated Qianlietongqiao Capsule can promote apoptosis of the detrusor muscle cells and evidently reduce the expression of NGF and bFGF in detrusor in benign prostatic hyperplasia（BPH） rats. And it has been reported in the literature that the persistent bladder outlet obstruction（BOO）induced by BPH can result in decreased blood flow and hypoxia in the bladder.Nevertheless，whether or not Qianlietongqiao Capsule can regulate bladder blood flow（BBF）and mitigate hypoxia of bladder after BOO remains undefined.And the above-mentioned is a key scientific problem which needs to be resolved.To reveal the targets and mechanism of Qianlietongqiao Capsule further，we will creat partial BOO rat models through the ligation of prostatic urethra using polypropylene suture and BOO rats are treated with drugs by gavage.Then the Fluorescent Microspheres Technique for determining BBF is requisite.And we will isolate and culture detrusor smooth muscle cells（DSMCs）of SD rats using collagenase digestion method.The hypoxia models of DSMCs will be established in a hypoxic chamber in which DSMSs will be treated with Qianlietongqiao Capsule-containing serum.DSMCs’ viability and the expression of HIF，VEGF，TGF-β，TNF-α and so forth in the bladder and DSMCs are determined by immunohistochemistry technique，Western Blot and RT-PCR.Through this project，partial mechanism of Qianlietongqiao Capsule used for protecting bladder function after bladder outlet obstruction will be uncovered and meanwhile it lays a theoretical basis for the further develop of new Chinese patent medicine which will be used for treating BOO-induced bladder dysfunction.