为维持正常的生理活动，蛋白质一直处于合成与降解的动态平衡过程中，因而其稳定性的调控对于生命体正常活动有着至关重要的作用。在茉莉素信号通路中，F-box蛋白COI1作为茉莉素的受体，调控茉莉素介导的植物生长发育过程及对外界伤害的防御反应。因此，COI1蛋白的稳定性调控对于植物正常生长发育具有重要的调控作用。本实验室的前期工作已初步揭示植物体内SCF复合体稳定COI1蛋白，而泛素修饰及26S蛋白酶体则降解COI1蛋白，这种动态平衡维持植物体内COI1蛋白的稳定性。然而，迄今为止，COI1蛋白的降解机制并不清楚。本项目将基于前期扎实的研究基础上，采用生化与正向遗传学方法，分离介导COI1降解的E3连接酶或相关调控因子，从而揭示F-box蛋白COI1的降解机制。COI1蛋白降解机制的揭示不仅对于剖析苿莉素信号途径具有重要价值，对于其他F-box蛋白的稳定性调控研究也具有普遍的生物学意义。

In cells, protein is always in a dynamic equilibrium process of synthesis and degradation, so its stability is very important for the organism activity. In jasmonate signal pathway, the F-box protein COI1, as jasmonate receptor, plays a vital role in plant development and defense, therefore its stability is essential for plant. In previous study，we have demonstrated that COI1 is stabilized by integrity of SCF complex and degraded via 26S proteasome pathway, which maintains stability of COI1 in protein level. However, the degradation mechanism of COI1 protein is still unknown. Based on the previous results, we want to identify the E3 ligase or other related regulators for COI1 degradation via forward genetic screen and biochemistry experiments. To reveal the degradation mechanism of COI1 protein not only has important value for the analysis of jasmonate signal pathway but also has biological significance for the study of the stability of other F-box proteins.