基于“肾主生殖”理论研究补肾活血法调控小鼠子宫内膜CFTR-ENaC离子通道的作用机制

胚胎种植成功是妊娠的先决条件，但由于子宫内膜容受性的下降，ART的胚胎种植率水平较低。课题组基于前期研究结果发现，补肾活血法可以通过调节围种植期内膜容受性相关因子及uNK旁分泌系统提高内膜容受性，促进妊娠。但子宫内膜容受性是受多个层面的时间-空间限制的复杂过程。除了课题组前期研究所涉及的方向，近期的研究表明，围种植期子宫腔内液体容量的规律变化是胚胎种植必要的空间结构形态学基础，促排卵治疗可以导致宫腔内异常积水从而降低妊娠率，这与课题组先前的研究发现不谋而合。课题组结合学科新进展，以促排卵小鼠模型为实验对象，采用磁共振成像及分子生物学技术，探讨补肾活血法对围种植期宫腔内液体容量、血清E2和P，以及CFTR、ENaC通道因子的调节作用；建立小鼠子宫内膜体外灌流模型，引入尤斯灌流系统，测量离子通道的短路电流及通路活性，拟从围种植期子宫空间结构形态学角度，阐述补肾活血法改善子宫内膜容受性的机制。

Embryo implantation is the precondition of pregnancy. Due to the decline in the receptivity of endometrium, implantation rate is very low. Based on our previous research results, we found that kidney nourishing and blood activating therapy adjust the endometrial receptivity related factors and uNK paracrine system. But the receptivity of endometrium is restricted by multiple levels of time - space complex process. In addition to the early stage of the research, recent research suggests that around the time of planting, the regular change of the liquid volume in the uterine cavity is embryonic planting the necessary space structure morphology, and stimulate ovulation treatment can lead to abnormal water reducing in uterus, which coincides with previously found. With new progress, ovulation model mice as the experimental object, we use MRI and molecular biology technology, to discuss the TCM control the intrauterine liquid capacity around the time of implanting, serum E2 and P, and CFTR, ENaC expression. Mice endometrial perfusion model in vitro is established, we using the Ussing chamber to measure short-circuit current in ion channel and channel activity. We propose the mechanism of kidney nourishing and blood activating therapy improves the receptivity of endometrium from the perspective of endometrial structure morphology.