解析持久抗条锈小麦品种的抗病性机理、发掘新的抗病基因，提高品种抗病持久性，是解决我国条锈病源头区域甘肃陇南小麦品种抗性易“丧失”的重要途径。陇南小麦品种兰引1号兼具苗期和成株期抗病性，抗病性保持约22年未变。申请人所在课题组前期进行品种成株抗条锈性全基因组关联分析发现兰引1号含有多个抗性QTL。本项目拟在此基础上，利用高通量SNP芯片和混池转录组测序（BSR-Seq)技术，进一步对兰引1号苗期和成株期抗条锈性基因解析：精细定位抗病基因和抗性QTL，发掘育种实用性分子标记，在抗病基因/QTL置信区间发掘候选抗病基因序列，鉴别引起抗病基因的因果突变位点。预期结果将加深人们对小麦持久抗病性分子机理的理解，并为持久抗病性小麦分子育种提供重要基因和标记。

Understanding the molecular basis of durable resistance to stripe rust in wheat cultivar, and identifying novel resistance genes to improve cultivar and deployment of them in wheat production, is an important strategy for solving the easily susceptible to the rust pathogen in wheat in South of Gansu, a hotspot for stripe rust in China. Wheat cultivar Lanyin 1 conferred durable resistance to stripe rust in both seedling and adult-plant stage during 22 years in wheat-growing region of Southern Gansu. Recently, using genome-wide association analysis (GWAS), we found multiple adult-plant resistance (APR) QTLs to stripe rust in cultivar Lanyin 1. Based on these results, this project is designed to understand the molecular mechanism of the resistance to stripe rust in cultivar Lanyin 1 by fine mapping the resistance genes and QTLs, and identifying the causative mutations in candidate resistance genes in confidence interval of QTL and finally find markers closely linked to these resistant genes for molecular breeding via SNP chip and Bulk Segregant RNA-Seq (BSR-Seq). This study is expected to deepen our understanding on molecular basis of durable resistance to stripe rust in wheat and provide important genes on improvement resistance of cultivars through molecular breeding.