Genetic Analysis of Adult Plant Resistance to Stripe Rust in Common Wheat Cultivar "Pascal".
Genetic Analysis of Adult Plant Resistance to Stripe Rust in Common Wheat Cultivar "Pascal".
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普通小麦品种“帕斯卡”成株抗条锈病的遗传分析
DOI：
10.3389/fpls.2022.918437
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发表时间：
2022
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
--
作者：
小麦条锈病是影响全球小麦产量的重要叶部病害。选育抗性小麦品种是控制这种病害最经济和最环保的方法之一。普通小麦（Triticum aestivum L.）品种“Pascal”在苗期对条锈病表现出易感性，但在中国西北病害重灾区甘肃，20多年来对条锈病表现出较高的抗性。为了解该品种抗条锈病的遗传机制，采用55K SNP芯片对“辉鲜红”ד帕斯卡”杂交的两个亲本和220个重组自交系（RIL）进行分析。我们检测到了Pascal贡献的三个新的条锈病成体抗性（APR）数量性状位点（QTL），即。 QYr.gaas-1AL、QYr.gaas-3DL和QYr.gaas-5AS，使用包含复合区间映射方法。它们的两侧分别是 SNP 标记 AX-111218361—AX-110577861、AX-111460455—AX-108798599 和 AX-111523523—AX-110028503，并解释了 11.0% 至 23.1% 的表型变异。使用批量分离外显子组捕获测序 (BSE-Seq) 对 QYr.gaas-1AL 进行精细定位，并选择 QYr.gaas-1AL 的候选基因 TraesCS1A02G313700、TraesCS1A02G313800 和 TraesCS1A02G314900。 QYr.gaas-1AL 和 QYr.gaas-3DL 的 KASP 标记 BSE-1A-12 和 HXPA-3D 是为育种者开发持久的抗条锈病小麦品种而开发的。
Wheat stripe rust is an important foliar disease that affects the wheat yield globally. Breeding for resistant wheat varieties is one of the most economically and environmentally effective ways to control this disease. The common wheat (Triticum aestivum L.) cultivar “Pascal” exhibited susceptibility to stripe rust at the seedling stage but it showed high resistance to stripe rust at the adult plant stage over 20 years in Gansu, a hotspot of the disease in northwestern China. To understand the genetic mechanism of stripe rust resistance in this cultivar, a 55K SNP array was used to analyze the two parents and the 220 recombinant inbred lines (RILs) derived from the cross of “Huixianhong” × “Pascal.” We detected three new stripe rust adult plant resistance (APR) quantitative trait locus (QTL) contributed by Pascal, viz. QYr.gaas-1AL, QYr.gaas-3DL, and QYr.gaas-5AS, using the inclusive composite interval mapping method. They were flanked by SNP markers AX-111218361—AX-110577861, AX-111460455—AX-108798599, and AX-111523523—AX-110028503, respectively, and explained the phenotypic variation ranging from 11.0 to 23.1%. Bulked segregant exome capture sequencing (BSE-Seq) was used for fine mapping of QYr.gaas-1AL and selection of candidate genes, TraesCS1A02G313700, TraesCS1A02G313800, and TraesCS1A02G314900 for QYr.gaas-1AL. KASP markers BSE-1A-12 and HXPA-3D for QYr.gaas-1AL and QYr.gaas-3DL were developed for breeders to develop durable stripe rust-resistant wheat varieties.
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在墨西哥环境下，在六倍层小麦线UC1110和PI610750中叶片锈和条纹抗锈蚀位点的表征和映射。
影响因子：
5.6
作者：
Lan C;Hale IL;Herrera-Foessel SA;Basnet BR;Randhawa MS;Huerta-Espino J;Dubcovsky J;Singh RP
通讯作者：
Singh RP
影响因子：
3.8
作者：
Chatterjee, A;Moulik, SP;Sanyal, SK
通讯作者：
Sanyal, SK
影响因子：
4.2
作者：
Draz, Ibrahim S.;Serfling, Albrecht;Roeder, Marion S.
通讯作者：
Roeder, Marion S.
影响因子：
56.9
作者：
Krattinger, Simon G.;Lagudah, Evans S.;Keller, Beat
通讯作者：
Keller, Beat
影响因子：
2.4
作者：
Escamez S;André D;Zhang B;Bollhöner B;Pesquet E;Tuominen H
通讯作者：
Tuominen H