生殖干细胞是人类和动物生殖系统的源头，在种族延续、配子发生等生命过程中扮演着重要的角色，对生殖干细胞的深入研究为治疗不孕不育以及攻克睾丸癌、卵巢癌等疾病，提供了重要的理论依据和技术支持。果蝇的雌性生殖干细胞系统（卵巢）是研究生殖干细胞命运决定机制非常理想的系统之一。申请人拟选择果蝇生殖干细胞为实验模型，以生殖干细胞的命运决定调控机理为研究方向，期望阐明SCF蛋白复合体调控分化因子Bam，从而决定果蝇生殖干细胞命运的分子机制。本项目的成功实施，将在国际上首次揭示蛋白翻译后修饰过程调控生殖干细胞分化因子Bam的分子机理，回答一个几十年来领域内共同努力却一直未解决的重要科学问题。本项目研究成果也将对高等动物干细胞命运决定调控机制的研究起到推动作用，进而促进临床上不孕不育治疗技术的革新和药物新药的开发。

Germline stem cells (GSCs), which are the headstreams of the reproductive systems of both human beings and animals, play pivotal roles in biological processes like race continuation and gametogenesis, etc. Fundamental studies on GSCs provide important theoretical basis and technical supports to the medical remedies of infertility, testicular and ovarian cancers. The Drosophila female reproductive system (ovaries) is one of the most ideal systems to study the molecular mechanisms of GSCs fate determination. We would like to utilize the Drosophila GSCs as study models and investigate how SCF complex determines the fate of GSCs through targeting the differentiating factor Bam. This project will be the first to uncover the molecular mechanisms by which Bam is modulated via protein post-translation modification in the world, thus solving an important scientific question that is studied by all the scientists in this field but still remains illusive. What's more, the conclusions of this study will positively contribute to the studies on the molecular mechanisms of mammalian GSCs fate determination, and the developments of new clinical technologies as well as medical drugs on the treatments of infertility.