5-HT系统改变已证实是情志致病及其从肝论治中枢机制的重要通路，但作为其合成释放的关键脑区中缝背核、并控制5-HT释放效应的突触前膜上5-HT1A自身受体负反馈作用及其与5-HT重摄取间的相互作用尚未得到深入研究。更重要的是，5-HT是目前青少期应激抑郁发生的唯一较为肯定通路并影响至成年，这且与中医学青少期脏腑“肝常有余”生理病理特性相吻合，提示从青少期5-HT通路入手研究调肝治法方药抗应激损伤中枢5-HT机制将是一个更加“明确”的途径。由此，本课题在前期海马5-HT通路研究的基础上，通过动物实验及细胞培养等方法，拟从形态、含量、信号传导等方面开展青少期应激大鼠突触前膜5-HT1A受体与5-HTT改变及调肝方药加味四逆散干预机制的研究，以期从“中缝背核-海马作用通路；合成-释放-调控环节”完整阐释调肝治法5-HT中枢机制。本研究亦为寻求青少期情志异常的中医有效干预手段提供依据。

Changes of 5-HT system have been proved to be the important pathways of central mechanism of emotional pathopoiesis and the treatment based on the liver, but the dorsal raphe nucleus which acts as a key brain region of its synthesis and release has not been studied. The dorsal raphe nucleus also controls the negative feedback effects of 5-HT1A receptor itself on 5-HT releasing in presynaptic membrane and its interaction with the reuptake of 5-HT. More importantly than all of that,traditional Chinese medicine holds that "the liver is often enough" is the physiological and pathological characteristics of the youth. The level of 5-HT in adolescence is already mature and maintaines to adulthood, and it is the only relatively sure access to stress-induced depression in youth period. All of these suggest that begin with the research of regulating liver therapy and prescription on anti-stress damage mechanism of central 5 - HT from the 5-HT pathway in adolescence may be a more "stable" way. Based on our previous studies on 5-HT pathways in hippocampal, we try to carry out the changes of presynaptic membrane 5- HT1A receptor and 5-HTT in adolescent rats under stress and intervention mechanism of Jiaweisinisan (Gan-regulating prescription) from the form, content, transmission of information and other aspects by the way such as cell culture and animal experiments. We expect to give a full interpretation of the 5-HT central mechanism of regulating liver therapy form the aspects of “dorsal raphe nucleus - hippocampus pathways and synthesis -release – regulation”. The study may also provide a basis for seeking effective interventions of TCM in adolescent sentiment abnormality.