目前，提高细毛羊质量与产量是细毛羊育种工作的重要任务，羊毛细度，净毛量，羊毛长度等性状是影响羊毛质量和产量的重要因素。为此，对这些羊毛性状的选育，对提高羊毛质量和产量有着重要的实际意义。本研究拟在已完成超细型细毛羊皮肤组织cDNA文库的构建的基础上,克隆和鉴定一批新基因；采用实时荧光定量PCR技术SYBR Green I法，以18S，ACTB，GAPDH为内参基因通过对不同细度绵羊皮肤组织的基因表达差异进行比较研究,探讨差异表达基因在不同细度绵羊皮肤组织中的表达规律；选择差异表达基因作为侯选基因，在基因组DNA水平上研究其遗传调控效应，为细毛羊毛纤维性状的改良进一步提供依据。

At present, improving fine-wool sheep quality and yield is the important task of fine-wool sheep breeding. The traits of the fineness of wool, the clear wool yield, wool length are important factors to affect wool quality and yield. Therefore, breeding for these wool traits to improve wool quality and yield are practical significance. This study has completed the superfine-wool sheep skin cDNA library and will clone and identification of a number of new genes; using real-time fluorescence quantitative PCR-SYBR Green I method, 18S, ACTB and GAPDH as reference gene through gene expression differences in the fineness of sheep skin tissue research. Investigate the differential expression of the gene expression pattern in different fineness sheep skin tissue; select differentially expressed genes as candidate genes to study the effect of their genetic regulation, and further provide the basis for the fine-wool sheep wool fiber trait improvement.