脑梗死是神经系统的常见病和多发病，致残率高。遗留运动、感觉、认知等功能障碍。循经按摩治疗本病具有较好的疗效，但其机制有待进一步明确。基于文献及前期研究，提出：循经按摩作用于肌肉组织，通过调节肌肉组织miR-206表达，进而促进肌肉BDNF表达，促进神经肌肉接头突触传递效率，促进运动神经元的存活，重建受损神经，改善运动功能的假说。为此，课题组拟运用循经按摩治疗脑缺血大鼠模型，观察其对脑梗死大鼠运动功能恢复、缺血区神经细胞超微结构及骨骼肌miR-206、BDNF的影响，并结合分子生物学技术，靶向增强、抑制C2C12成肌细胞miR-206表达，观察其对BDNF的影响。探讨骨骼肌miR-206调控BDNF在循经按摩中的作用，揭示循经按摩促进脑梗死后运动功能重建的机制。

Cerebral infarction is a common and frequent disease of the nervous system,high morbidity,easily resulting in motor, sensory and cognitive dysfunction.Meridian massage showed better efficacy,but its mechanism remains to be further defined.Based on the literature and preliminary studies,the following hypothesis was presented:the role of meridian massage can work on muscle tissue, by regulating the expression of miR-206, further promote muscle BDNF expression,thereby contribute to functional changes of synapses, finally lead to rebuilding damaged nerve function. Therefore,the research group intended to treat rats model of cerebral ischemia by using meridian massage, which effect would be observed on movement function recovery, and on neuronal cell ultrastructure in ischemic zone, as well as on skeletal muscle miR-206 and BDNF.In addition,combined with molecular biology techniques,the effect on BDNF targeting at enhancement or inhibition the expression of C2C12 myoblasts miR-206 also was observed.This research investigated the regulation of skeletal muscle miR-206 mediating BDNF in the meridian massage, and revealed the mechanism of rehabilitation in promoting the recovery of movement function after cerebral infarction.