膀胱癌发病率位居泌尿系统肿瘤首位，肿瘤转移是造成其死亡的主要原因，实现膀胱癌转移的早期诊断有助于指导临床治疗，但目前缺乏特异性指标。研究发现，lncRNA参与调控肿瘤细胞的侵袭转移，有望用于膀胱癌转移的诊断，但其游离在血液中易降解；Exosomes是细胞分泌的微小囊泡，lncRNA可“储藏”其中从而在外周血中保持稳定。我们前期通过lncRNA芯片发现，膀胱癌患者外周血Exosomes中lncRNA表达与肿瘤组织中基本一致，两者均与癌旁组织存在显著差异。本课题拟利用lncRNA芯片技术，通过膀胱癌临床标本系统分析获得可用于其转移早期诊断的外周血Exosomes中lncRNA表达谱型；进一步通过体内外实验证实这些lncRNAs对膀胱癌转移的调控作用，并借助ChIRP-Seq、ChIRP-MS等技术筛选其直接作用的靶基因和靶蛋白，明确其作用机制。研究结果可望为膀胱癌转移的早期诊治提供全新指标。

The incidence of bladder cancer ranks first in the urological tumor. Cancer metastasis is the leading cause of tumor related deaths in patients with bladder cancer. The early diagnosis of metastatic bladder cancer can help guide clinical treatment, which is restricted to the lack of specific indicators. LncRNA is recognized to be involved in the cancer metastasis, which is promising in the diagnosis of metastatic bladder cancer. However, lncRNA is easy to be degraded in peripheral blood. Exosomes are small vesicles secreted by cells in which LncRNAs are encapsulated, and thus to protect LncRNAs from degradation. The expression profile of lncRNA in exosomes of bladder cancer patients was correlated to that in bladder cancer tissues, both were significantly different from the lncRNA expression in the paraneoplastic tissues. In the current study, we want to obtain the circulating exosomal lncRNA expression profile via high throughput lncRNA microarray using a large number of bladder cancer specimens, which can be used for the early diagnosis of metastatic bladder cancer. Furthermore, in vivo and in vitro experiments are designed to verify the role of these lncRNAs in regulating bladder cancer metastasis, and ChIRP-Seq, ChIRP-MS and other techniques will be applied to identify the target protein/target gene and further reveal the underlying mechanism. This study will provide new promising indicators for the early diagnosis and treatment of bladder cancer metastasis.